biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsSeongmin KimIllumina NovaSeq 6000DNA-seqCanis lupus familiarisCanis lupus familiarishttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14410Solid tumors were histopathologically categorized into carcinomas and sarcomas and analyzed based on structural variations specific to each group. To investigate this, we utilized whole genome sequencing (WGS) data from dogs with solid tumors, including carcinomas and sarcomas. Structural variations, which induce extensive base changes, significantly impact tumor development. Through our analysis, we identified 146,847 structural variations within the solid tumor data. By comparing each solid tumor group, we identified both common genes affecting tumors across groups and differential genes unique to carcinomas and sarcomas. This finding suggests that understanding the specific genetic alterations associated with each tumor type can enhance the accuracy of diagnosis, inform treatment strategies, and improve prognostic assessments.biostudies-arrayexpressSample Collection - Four types of cancer tissues were collected from four different dogs. Urothelial carcinoma tissue was excised post-mortem from the bladder to the urethra of a 12-year-old female Dachshund, following confirmation of whole-body metastasis via CT. Intestinal adenocarcinoma tissue was obtained during therapeutic resection of an intestinal mass in an 11-year-old neutered male Spitz at the time of diagnosis. Nasal chondrosarcoma was diagnosed in a 5-year-old male Poodle, with tissue collected through a Tru-Cut biopsy after a CT scan of the nasal cavity. Rhabdomyosarcoma tissue was collected via Tru-Cut biopsy from a right hindlimb mass in an 8-year-old male Borzoi after anesthesia. In each case, a tissue sample measuring approximately 1 cm × 1 cm was excised, immediately stored at -80°C.Nucleic Acid Extraction - Genomic DNA was extracted from each tumor tissue using a QIAamp Blood & Tissue Kit (QIAGEN, Hilden, Germany).Library Construction - Individual libraries were generated using Illumina TruSeq DNA PCR-Free Kit (Illumina, San Diego, CA, USA).Sequencing - Whole-genome sequencing was performed using the Illumina NovaSeq 6000 sequencer. (Illumina, San Diego, CA, USA).OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesSeongmin KimfalseCharacterization of structural variants in canine carcinoma and sarcomaSolid tumors were histopathologically categorized into carcinomas and sarcomas and analyzed based on structural variations specific to each group. To investigate this, we utilized whole genome sequencing (WGS) data from dogs with solid tumors, including carcinomas and sarcomas. Structural variations, which induce extensive base changes, significantly impact tumor development. Through our analysis, we identified 146,847 structural variations within the solid tumor data. By comparing each solid tumor group, we identified both common genes affecting tumors across groups and differential genes unique to carcinomas and sarcomas. This finding suggests that understanding the specific genetic alterations associated with each tumor type can enhance the accuracy of diagnosis, inform treatment strategies, and improve prognostic assessments.2025-12-01T00:00:00Z2025-12-01T02:01:49.742Z2024-09-03T16:58:29.785ZE-MTAB-14410ERP163770EFO_0002944EFO_0004170EFO_0002693EFO_0005518EFO_0004184