{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":[null],"organism":["Arabidopsis thaliana"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14424"],"description":["This experiment aims to analyze crossover distribution in Arabidopsis female and male meiosis. The heterozygous mutant plants in Col-0 and Ler were crossed as females and males respectively. Wild-type and homozygous mutant plants were selected among the F1s and crossed as female or as male with wild-type Col-0. Leaf samples from the four back-crossed populations were used for DNA purification and library preparation for Illumina sequencing (NextSeq 2000 2×150 base pairs [bp]), performed at the Max Planck-Genome-center (https://mpgc.mpipz.mpg.de/home/)."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - Leaves were removed from plants and stored at -80 C until DNA extraction","Growth Protocol - Greenhouse supplemented with additional light. Plants grown in the greenhouse (16 h day/8 h night) for 3 weeks and 4 days in the dark","Library Construction - TPase-based DNA library (>95 samples)","Sequencing - Illumina NextSeq 2000","Nucleic Acid Extraction - NucleoMag Plant Kit (Macherey and Nagel, P/O 744400.4) on a robotic device (KingFisher, Thermo)"],"figure_sub":["MINSEQE Score","Assays and Data","organisation","MAGE-TAB Files"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["NextSeq 2000"],"study_type":["DNA-seq"],"species":["Arabidopsis thaliana"],"additional_accession":["E-MTAB-9593"],"pubmed_authors":["Qichao Lian","Raphael Mercier"]},"is_claimable":false,"name":"Whole-genome resequencing of BC1 populations from Arabidopsis (Col x Ler) x Col crosses (wt, zyp1, HEI10oe)","description":"This experiment aims to analyze crossover distribution in Arabidopsis female and male meiosis. The heterozygous mutant plants in Col-0 and Ler were crossed as females and males respectively. Wild-type and homozygous mutant plants were selected among the F1s and crossed as female or as male with wild-type Col-0. Leaf samples from the four back-crossed populations were used for DNA purification and library preparation for Illumina sequencing (NextSeq 2000 2×150 base pairs [bp]), performed at the Max Planck-Genome-center (https://mpgc.mpipz.mpg.de/home/).","dates":{"release":"2025-05-09T00:00:00Z","modification":"2026-06-05T11:00:23.6Z","creation":"2024-09-05T15:57:59.817Z"},"accession":"E-MTAB-14424","cross_references":{"ENA":["ERP163876"],"Biostudies":["E-MTAB-14427","E-MTAB-14426","E-MTAB-14428","E-MTAB-14423","E-MTAB-14422","E-MTAB-14425","E-MTAB-14430","E-MTAB-9593","E-MTAB-11696","E-MTAB-11254"],"EFO":["EFO_0002944","EFO_0004170","EFO_0003789","EFO_0002693","EFO_0005518","EFO_0004184"]}}