biostudies-arrayexpressArabidopsis thalianahttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14426This experiment aims to analyze crossover distribution in Arabidopsis female and male meiosis. The heterozygous mutant plants in Col-0 and Ler were crossed as females and males respectively. Wild-type and homozygous mutant plants were selected among the F1s and crossed as female or as male with wild-type Col-0. Leaf samples from the four back-crossed populations were used for DNA purification and library preparation for Illumina sequencing (NextSeq 2000 2×150 base pairs [bp]), performed at the Max Planck-Genome-center (https://mpgc.mpipz.mpg.de/home/).biostudies-arrayexpressSample Collection - Leaves were removed from plants and stored at -80 C until DNA extractionGrowth Protocol - Greenhouse supplemented with additional light. Plants grown in the greenhouse (16 h day/8 h night) for 3 weeks and 4 days in the darkLibrary Construction - TPase-based DNA library (>95 samples)Sequencing - Illumina NextSeq 2000Nucleic Acid Extraction - NucleoMag Plant Kit (Macherey and Nagel, P/O 744400.4) on a robotic device (KingFisher, Thermo)MINSEQE ScoreAssays and DataorganisationMAGE-TAB FilesMetabolomicsUnknownTranscriptomicsGenomicsProteomicsNextSeq 2000DNA-seqArabidopsis thalianaE-MTAB-9593Qichao LianRaphael MercierfalseWhole-genome resequencing of BC1 populations from Arabidopsis (Col x Ler) x Col crosses (recq4ab_HEI10oe)This experiment aims to analyze crossover distribution in Arabidopsis female and male meiosis. The heterozygous mutant plants in Col-0 and Ler were crossed as females and males respectively. Wild-type and homozygous mutant plants were selected among the F1s and crossed as female or as male with wild-type Col-0. Leaf samples from the four back-crossed populations were used for DNA purification and library preparation for Illumina sequencing (NextSeq 2000 2×150 base pairs [bp]), performed at the Max Planck-Genome-center (https://mpgc.mpipz.mpg.de/home/).2025-05-09T00:00:00Z2026-06-05T11:00:27.033Z2024-09-05T16:04:51.419ZE-MTAB-14426ERP163879E-MTAB-14427E-MTAB-14428E-MTAB-14423E-MTAB-14422E-MTAB-14425E-MTAB-14424E-MTAB-14430E-MTAB-9593E-MTAB-11696E-MTAB-11254EFO_0002944EFO_0004170EFO_0003789EFO_0002693EFO_0005518EFO_0004184