{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Andreas Bikfalvi"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["single nucleus RNA sequencing"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14432"],"description":["Murine renal carcinoma RENCA cells were injected in BALB/c mice to generated either primary tumours (orthotopically) or lunge metastases (through tail vein injection, or directly forming from primary tumors). Samples were snap-frozen and processed for single nucleus isolation and RNA seq analysis. Tumor cells expressed Interleukin-34 (IL34-OE) or an empty vector as control (Ctrl). The goal is to finely dissect tumor microenvironmental changes occurring in an IL34-enriched tumor microenvironment, either in primary tumors or lung metastases."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - Fresh samples were quick-frozen in liquid nitrogen and stored at -80°C until the day of nuclei isolation","Library Construction - 17,000 nuclei/sample were processed for Gel Bead-in-Emulsion (GEM) and library generation using Chromium Next GEM Single Cell 3’ GEM, Library & Gel Bead Kit v3.1 (10X Genomics)","Sequencing - The libraries were sequenced using an Illumina NovaSeq 6000","Nucleic Acid Extraction - Frozen samples were dounce-homogenized in ice-cold Lysis Buffer containing 0.1% TritonX"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Andreas Bikfalvi","Andrea Emanuelli"],"additional_accession":[]},"is_claimable":false,"name":"Single-nucleus RNA seq of murine renal tumors and lung metastases generated by RENCA cells overexpressing Interleukin-34","description":"Murine renal carcinoma RENCA cells were injected in BALB/c mice to generated either primary tumours (orthotopically) or lunge metastases (through tail vein injection, or directly forming from primary tumors). Samples were snap-frozen and processed for single nucleus isolation and RNA seq analysis. Tumor cells expressed Interleukin-34 (IL34-OE) or an empty vector as control (Ctrl). The goal is to finely dissect tumor microenvironmental changes occurring in an IL34-enriched tumor microenvironment, either in primary tumors or lung metastases.","dates":{"release":"2025-06-10T00:00:00Z","modification":"2025-06-10T10:00:49.02Z","creation":"2024-09-06T12:21:42.388Z"},"accession":"E-MTAB-14432","cross_references":{"ENA":["ERP163889"],"EFO":["EFO_0002944","EFO_0004170","EFO_0009809","EFO_0005518","EFO_0004184"]}}