biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsAndreas BikfalviIllumina NovaSeq 6000single nucleus RNA sequencingMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14432Murine renal carcinoma RENCA cells were injected in BALB/c mice to generated either primary tumours (orthotopically) or lunge metastases (through tail vein injection, or directly forming from primary tumors). Samples were snap-frozen and processed for single nucleus isolation and RNA seq analysis. Tumor cells expressed Interleukin-34 (IL34-OE) or an empty vector as control (Ctrl). The goal is to finely dissect tumor microenvironmental changes occurring in an IL34-enriched tumor microenvironment, either in primary tumors or lung metastases.biostudies-arrayexpressSample Collection - Fresh samples were quick-frozen in liquid nitrogen and stored at -80°C until the day of nuclei isolationLibrary Construction - 17,000 nuclei/sample were processed for Gel Bead-in-Emulsion (GEM) and library generation using Chromium Next GEM Single Cell 3’ GEM, Library & Gel Bead Kit v3.1 (10X Genomics)Sequencing - The libraries were sequenced using an Illumina NovaSeq 6000Nucleic Acid Extraction - Frozen samples were dounce-homogenized in ice-cold Lysis Buffer containing 0.1% TritonXOrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesAndreas BikfalviAndrea EmanuellifalseSingle-nucleus RNA seq of murine renal tumors and lung metastases generated by RENCA cells overexpressing Interleukin-34Murine renal carcinoma RENCA cells were injected in BALB/c mice to generated either primary tumours (orthotopically) or lunge metastases (through tail vein injection, or directly forming from primary tumors). Samples were snap-frozen and processed for single nucleus isolation and RNA seq analysis. Tumor cells expressed Interleukin-34 (IL34-OE) or an empty vector as control (Ctrl). The goal is to finely dissect tumor microenvironmental changes occurring in an IL34-enriched tumor microenvironment, either in primary tumors or lung metastases.2025-06-10T00:00:00Z2025-06-10T10:00:49.02Z2024-09-06T12:21:42.388ZE-MTAB-14432ERP163889EFO_0002944EFO_0004170EFO_0009809EFO_0005518EFO_0004184