biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsData Submission IFOM - ETSIon Torrent ProtonRNA-seq of coding RNASchmidtea mediterraneaSchmidtea mediterraneahttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14438The aim of the experiment was to compare the transcriptome of intact control and prep knock down animals.biostudies-arrayexpressNucleic Acid Extraction - Animals were homogenized with a motorized pestle in Trizol reagent (Thermo Fisher Scientific) and total RNA extraction was performed following manufacturer’s protocol. Purified RNA was DNase treated (Promega) and column purified (Qiagene).Library Construction - For RNA sequencing, RNA integrity was assessed using Agilent Bioanalyzer 2100 with Nano Rna kit (RIN > 8). Libraries were prepared following the manufacturer protocol for transcriptome sequencing with the Ion Proton System (Thermo Fisher Scientific). mRNA-seq indexed library preparation was performed starting from 1 microg of total RNA with the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) according to the manufacturer’s instructions. Indexed libraries were quality controlled on Agilent Bioanalyzer 2100 with High Sensitivity DNA kit, quantified with Qubit HS DNA, normalized and pooled to perform a multiplexed sequencing run.Sequencing - Sequencing was performed in SE mode (1x150nt) on an Ion Proton platform, generating on average 12 millions SE reads per sample.Sample Collection - 20 animals per sample were collected 7 days after the last RNAi feeding.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesJelena VermezovicData Submission IFOM - ETSPaolo BonaiutifalseRNA sequencing from planarian tissuesThe aim of the experiment was to compare the transcriptome of intact control and prep knock down animals.2026-02-01T00:00:00Z2026-02-01T02:02:25.392Z2024-09-09T16:13:44.805ZE-MTAB-14438ERP163988EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184