biostudies-arrayexpressEllen De PauwMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14455Female cystic fibrosis (CF) patients are confronted with fertility deficiency, experiencing difficulties in getting pregnant. Previous findings using CF mouse models indicate that CF negatively impacts female fertility in mice as it does in humans. Underlying reasons remain elusive. In particular, it has only poorly been studied whether the endometrium, the inner lining of the womb and crucial tissue for embryo implantation, plays a role. Here, we applied single cell transcriptomics of wild-type (F508del <wt/wt>; WT) and CF (F508<del/del>) mouse uteri from the Cftr<tm1eur> mouse model, to get a detailed view on cellular and molecular/pathway differences in CF versus WT endometrium. Five uteri (WT: 3, CF: 2) were assessed in the estrus cycle of the estrous cycle, one CF uteri was sequenced during the diestrus cycle phase.biostudies-arrayexpressSample Collection - Estrous cycle phase was determined by vaginal smear.14-15 weeks old mice were euthanized by CO2 asphyxiation, followed by uterine horn dissection. The uterine horns were longitudinally opened to expose the endometrium and cut into small pieces. Subsequently, these uterine horn pieces were dissociated enzymatically in 1% collagenase for 60 minutes at 37°C. Afterwards, the endometrial tissue pieces were mechanically dissociated by pipetting up and down for 10 minutes. 10% FBS was added to stop the enzymatic reaction. The cell suspension was filtered through a 40 μm cell strainer.Nucleic Acid Extraction - The uterus single-cell suspensions were converted to barcoded Drop-seq libraries by using the Chromium Single Cell 3’ V3.1 Library, Gel Bead & Multiplex Kit and Chip Kit (10X Genomics; Pleasanton, California, USA), aiming for an estimated 8,000 cells per library.Library Construction - Single-cell libraries were prepared with the Chromium Single Cell 3’ V3.1 Chemistry Library Kit, Gel Bead & Multiplex Kit and Chip Kit from 10X Genomics.Sequencing - The barcoded library was sequenced on an Illumina Nextseq and NovaSeq6000 in 25-8-98 paired-end configuration up to a saturation over 60%.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNA from single cellsMus musculusEndometrium-derived organoids from cystic fibrosis patients and mice as new models to study disease-associated endometrial pathobiologyDiether LambrechtsCéline BuedsBeau GommersEllen De PauwHugo VankelecomSilke De VriendtEllen De Pauw, Beau Gommers, Marjolein M. Ensinck, Stefan Timmerman, Silke De Vriendt, Celine Bueds, Mengjie Wei, Florian Hermans, Kaline Arnauts, Anabela Santo Ramalho Venâncio, François Vermeulen, Lieven Dupont, Diether Lambrechts, Marianne S. Carlon, Hugo VankelecomfalseSingle-cell transcriptomics of wild-type and cystic fibrosis (F508del/del) mouse uterusFemale cystic fibrosis (CF) patients are confronted with fertility deficiency, experiencing difficulties in getting pregnant. Previous findings using CF mouse models indicate that CF negatively impacts female fertility in mice as it does in humans. Underlying reasons remain elusive. In particular, it has only poorly been studied whether the endometrium, the inner lining of the womb and crucial tissue for embryo implantation, plays a role. Here, we applied single cell transcriptomics of wild-type (F508del <wt/wt>; WT) and CF (F508<del/del>) mouse uteri from the Cftr<tm1eur> mouse model, to get a detailed view on cellular and molecular/pathway differences in CF versus WT endometrium. Five uteri (WT: 3, CF: 2) were assessed in the estrus cycle of the estrous cycle, one CF uteri was sequenced during the diestrus cycle phase.2025-10-31T00:00:00Z2025-10-31T02:01:44.324Z2024-09-18T14:11:26.922ZE-MTAB-14455ERP164320E-MTAB-14456EFO_0002944EFO_0004170EFO_0005684EFO_0005518EFO_0004184