{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Chew Weng Cheng"],"instrument_platform":["Illumina HiSeq 2500","TriZol reagent","Illumina"],"study_type":["RNA-seq of coding RNA"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14458"],"description":["The aim of the project is to investigate whether endothelial-restricted augmentation of IGF-1 signaling is sufficient to suppress atherogenesis. We generated RNA-seq data from mice with endothelial-restricted over-expression of human wildtype IGF-1R (hIGFREO/ApoE-/-) or a signaling defective K1003R mutant human IGF-1R (mIGFREO/ApoE-/-)."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - We generated mice with endothelial-restricted over-expression of human wildtype IGF-1R (hIGFREO/ApoE-/-) or a signaling defective K1003R mutant human IGF-1R (mIGFREO/ApoE-/-)","Nucleic Acid Extraction - Total RNA was prepared from tissues using TriZol reagent (Sigma).","Sequencing - The qualified libraries are fed into Illumina sequencers after pooling according to its effective concentration and expected data volume. The samples were sequenced using a 1x75bp single-end configuration.","Library Construction - RNA sequencing was performed by sequencing facilities at the University of Leeds. After the QC procedures, mRNA from eukaryotic organisms is enriched using oligo(dT) beads. First, the mRNA is fragmented randomly by adding fragmentation buffer, then the cDNA is synthesized by using mRNA template and random hexamers primer, after which a custom second-strand synthesis buffer (Illumina) , dNTPs, RNase H and DNA polymerase I are added to initiate the second-strand synthesis. Second, after a series of terminal repair, A ligation and sequencing adaptor ligation, the double-stranded cDNA library is completed through size selection and PCR enrichment."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Richard Cubbon","Chew Weng Cheng"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of IGF-1R in atherosclerosis","description":"The aim of the project is to investigate whether endothelial-restricted augmentation of IGF-1 signaling is sufficient to suppress atherogenesis. We generated RNA-seq data from mice with endothelial-restricted over-expression of human wildtype IGF-1R (hIGFREO/ApoE-/-) or a signaling defective K1003R mutant human IGF-1R (mIGFREO/ApoE-/-).","dates":{"release":"2025-07-31T00:00:00Z","modification":"2024-09-19T09:10:00.532Z","creation":"2024-09-19T09:10:00.532Z"},"accession":"E-MTAB-14458","cross_references":{"ENA":["ERP164323"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}