{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Shan Fang"],"instrument_platform":["Illumina HiSeq 2500"],"study_type":["RNA-seq of coding RNA"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14467"],"description":["Whole blood samples from 10 healthy controls and 10 acute ichemic stroke patients"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - The training cohort comprises 1 mL of whole blood samples from 10 HC and 10 AIS groups, while the validation cohort consists of an additional 10 HC and 10 AIS groups.","Nucleic Acid Extraction - Enrichment of polyA-tailed mRNA is achieved by using Oligo(dT) magnetic beads. Subsequently, the RNA is fragmented into short pieces by adding fragmentation buffer. These short RNA fragments serve as templates for the synthesis of the first-strand cDNA using random hexamers. The second-strand cDNA is synthesized by adding buffer, dNTPs (dTTP, dATP, dGTP, and dCTP), and DNA polymerase I. The double-stranded cDNA is then purified using DNA purification magnetic beads.","Library Construction - Following purification, the double-stranded cDNA undergoes end-repair, adenylation of the 3' ends, and ligation of sequencing adapters. DNA purification magnetic beads are used again for size selection of the cDNA fragments. Finally, PCR amplification is performed to enrich and obtain the final cDNA library.","Sequencing - After the construction of the library is complete, its quality must be assessed to ensure it meets the requirements before proceeding to sequencing. The quality assessment is conducted as follows:  1. Preliminary quantification is performed using the Qubit fluorometric method, and the insert size of the library is analyzed using a fragment analyzer. Only when the insert size is as expected can the next step be initiated.  2. The effective concentration of the library is accurately quantified using the qPCR method (with an effective concentration greater than 2 nM) to complete the library check. Once the library check is passed, different libraries are pooled according to the target sequencing data volume and sequenced using the Illumina platform."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Shan Fang"],"additional_accession":[]},"is_claimable":false,"name":"High-throughput sequencing of whole blood samples from 10 healthy controls and 10 acute ichemic stroke patients","description":"Whole blood samples from 10 healthy controls and 10 acute ichemic stroke patients","dates":{"release":"2025-10-01T00:00:00Z","modification":"2025-10-01T01:06:00.361Z","creation":"2024-09-20T11:46:58.461Z"},"accession":"E-MTAB-14467","cross_references":{"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}