{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Yong Zhou"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14480"],"description":["We undertook single-nucleus RNA sequencing to analyze primitive hematopoietic progenitors that circulate in embryonic mouse heart tubes at E10.5."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - E10.5 embryonic hearts was dissected and quickly frozen in liquid nitrogen to retain intact nuclei.","Library Construction - The sc-RNA-seq libraries were generated using Chromium Single Cell 3’ Reagent Kits v2 and quantified using a Bioanalyzer 2200.","Nucleic Acid Extraction - After nuclei extraction and purification, approximately 15,000 nuclei per sample were loaded on a Chromium Controller (10x Genomics) to generate gel bead-in-emulsions.","Sequencing - All libraries were sequenced by Novaseq6000 (Illumina) on a 150 bp paired-end run."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - Illumina NovaSeq6000 RTA (v.3.4.4) software was used to perform basecalling, and the resulting base call files (.bcl) were converted to standard fastq formatted sequence files using Bcl2Fastq (v.2.20, Illumina). Feature-barcode matrices were obtained by aligning reads to the crab-eating macaque genome (Mfa5.0) using CellRanger v.3.0.0."],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Bioanalyzer 2200","NA","Illumina NovaSeq 6000","Chromium Controller"],"study_type":["single nucleus RNA sequencing"],"species":["Mus musculus"],"pubmed_authors":["Yong Zhou"],"additional_accession":[]},"is_claimable":false,"name":"Primitive erythroid and myeloid progenitors in mouse embryos","description":"We undertook single-nucleus RNA sequencing to analyze primitive hematopoietic progenitors that circulate in embryonic mouse heart tubes at E10.5.","dates":{"release":"2026-06-30T00:00:00Z","modification":"2026-06-30T01:00:54.257Z","creation":"2024-09-26T15:52:01.785Z"},"accession":"E-MTAB-14480","cross_references":{"ENA":["ERP164532"],"EFO":["EFO_0002944","EFO_0004170","EFO_0009809","EFO_0005518","EFO_0003816","EFO_0004184"]}}