biostudies-arrayexpressYong ZhouMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14480We undertook single-nucleus RNA sequencing to analyze primitive hematopoietic progenitors that circulate in embryonic mouse heart tubes at E10.5.biostudies-arrayexpressSample Collection - E10.5 embryonic hearts was dissected and quickly frozen in liquid nitrogen to retain intact nuclei.Library Construction - The sc-RNA-seq libraries were generated using Chromium Single Cell 3’ Reagent Kits v2 and quantified using a Bioanalyzer 2200.Nucleic Acid Extraction - After nuclei extraction and purification, approximately 15,000 nuclei per sample were loaded on a Chromium Controller (10x Genomics) to generate gel bead-in-emulsions.Sequencing - All libraries were sequenced by Novaseq6000 (Illumina) on a 150 bp paired-end run.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Illumina NovaSeq6000 RTA (v.3.4.4) software was used to perform basecalling, and the resulting base call files (.bcl) were converted to standard fastq formatted sequence files using Bcl2Fastq (v.2.20, Illumina). Feature-barcode matrices were obtained by aligning reads to the crab-eating macaque genome (Mfa5.0) using CellRanger v.3.0.0.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsBioanalyzer 2200NAIllumina NovaSeq 6000Chromium Controllersingle nucleus RNA sequencingMus musculusYong ZhoufalsePrimitive erythroid and myeloid progenitors in mouse embryosWe undertook single-nucleus RNA sequencing to analyze primitive hematopoietic progenitors that circulate in embryonic mouse heart tubes at E10.5.2026-06-30T00:00:00Z2026-06-30T01:00:54.257Z2024-09-26T15:52:01.785ZE-MTAB-14480ERP164532EFO_0002944EFO_0004170EFO_0009809EFO_0005518EFO_0003816EFO_0004184