{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Yaw Shin Ooi"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14485"],"description":["Unbiased forward genetic screen to identify host factors for Pteropine Orthoreovirus PRV3M"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - A pool of mutagenized IGROV-1 cells were generated using the Brunello CRISPR knockout library, which was designed to target human genome with four unique sgRNA per gene. The mutagenized IGROV-1 cells were subjected to pteropine orthoreovirus PRV3M infection. After a total of two weeks of live/dead selection, mutagenized cells resisting cytolytic infection of pteropine orthoreovirus PRV3M was harvested fand subjected to NGS analysis.","Nucleic Acid Extraction - Resistant cells were harvested from each sub-library, and subjected to total genomic DNA extraction using DNA mini kit (Qiagen) according to the manufacturer’s instructions","Sequencing - Illumina MiSeq (50bp run)","Library Construction - The sgRNA were amplified from gDNA in two-step PCR using Herculase II Fusion DNA Polymerase (Agilent). For first PCR, 20 ug of gDNA for each library was amplified for 16 cycles. For 2nd PCR, 1 reaction containing 5ul PCR product for each sub-library was amplified for 27 cycles, using Illumina indexed primers. PCR products were gel purified (Qiagen gel purification kit) and sequenced on Illumina MiSeq platform."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - NGS data was analyzed using MAGeCK."],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina MiSeq","BSL2 Laboratory"],"study_type":["DNA-seq"],"species":["Homo sapiens"],"pubmed_authors":["Yaw Shin Ooi"],"additional_accession":[]},"is_claimable":false,"name":"Genome-scale CRISPR knockout screen for Pteropine Orthoreovirus PRV3M","description":"Unbiased forward genetic screen to identify host factors for Pteropine Orthoreovirus PRV3M","dates":{"release":"2025-12-31T00:00:00Z","modification":"2025-12-31T02:02:12.854Z","creation":"2024-10-01T10:13:58.515Z"},"accession":"E-MTAB-14485","cross_references":{"ENA":["ERP164647"],"EFO":["EFO_0002944","EFO_0004170","EFO_0002693","EFO_0005518","EFO_0003816","EFO_0004184"]}}