{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":[null],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14552"],"description":["Regenerative capacities are very limited in adult mammals at the benefit of scarring. Mesenchymal stroma cells (MSCs), shared by all organs and tissues, represent a key element in maintaining tissue architecture integrity and repair processes. They display high phenotypic plasticity and represent a heterogeneous population. We hypothesize that in the very early steps of tissue repair, regenerative and non-regenerative processes are associated with distinct qualitative change in MSCs heterogeneity that determine the repair final outcomes."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - RNA were extracted using Chromium Next GEM Single Cell 3'Reagent Kits v3.1 (Dual Index)","Sample Collection - Mouse adipose tissue were dissociated and CD45-CD31- cells were FACS sorted 1 and 3 days post-injury.","Library Construction - Libraries were constructed using Chromium Next GEM Single Cell 3'Reagent Kits v3.1 (Dual Index)","Sequencing - Samples were pooled together to 20 pM concentration and sequenced with an Illumina NextSeq."],"figure_sub":["MINSEQE Score","Assays and Data","organisation","MAGE-TAB Files"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["NextSeq 500"],"study_type":["RNA-seq of coding RNA from single cells"],"species":["Mus musculus"],"additional_accession":["ERP165382"],"pubmed_authors":["Marielle Ousset"]},"is_claimable":false,"name":"scRNA-seq of CD45-CD31- mesenchymal stromal cells isolated from mouse adipose tissue after tissue lesion","description":"Regenerative capacities are very limited in adult mammals at the benefit of scarring. Mesenchymal stroma cells (MSCs), shared by all organs and tissues, represent a key element in maintaining tissue architecture integrity and repair processes. They display high phenotypic plasticity and represent a heterogeneous population. We hypothesize that in the very early steps of tissue repair, regenerative and non-regenerative processes are associated with distinct qualitative change in MSCs heterogeneity that determine the repair final outcomes.","dates":{"release":"2026-03-16T00:00:00Z","modification":"2026-03-16T12:51:02.929Z","creation":"2024-10-22T14:27:15.228Z"},"accession":"E-MTAB-14552","cross_references":{"ENA":["ERP165382"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005684","EFO_0005518","EFO_0004184"]}}