biostudies-arrayexpressMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14552Regenerative capacities are very limited in adult mammals at the benefit of scarring. Mesenchymal stroma cells (MSCs), shared by all organs and tissues, represent a key element in maintaining tissue architecture integrity and repair processes. They display high phenotypic plasticity and represent a heterogeneous population. We hypothesize that in the very early steps of tissue repair, regenerative and non-regenerative processes are associated with distinct qualitative change in MSCs heterogeneity that determine the repair final outcomes.biostudies-arrayexpressNucleic Acid Extraction - RNA were extracted using Chromium Next GEM Single Cell 3'Reagent Kits v3.1 (Dual Index)Sample Collection - Mouse adipose tissue were dissociated and CD45-CD31- cells were FACS sorted 1 and 3 days post-injury.Library Construction - Libraries were constructed using Chromium Next GEM Single Cell 3'Reagent Kits v3.1 (Dual Index)Sequencing - Samples were pooled together to 20 pM concentration and sequenced with an Illumina NextSeq.MINSEQE ScoreAssays and DataorganisationMAGE-TAB FilesMetabolomicsUnknownTranscriptomicsGenomicsProteomicsNextSeq 500RNA-seq of coding RNA from single cellsMus musculusERP165382Marielle OussetfalsescRNA-seq of CD45-CD31- mesenchymal stromal cells isolated from mouse adipose tissue after tissue lesionRegenerative capacities are very limited in adult mammals at the benefit of scarring. Mesenchymal stroma cells (MSCs), shared by all organs and tissues, represent a key element in maintaining tissue architecture integrity and repair processes. They display high phenotypic plasticity and represent a heterogeneous population. We hypothesize that in the very early steps of tissue repair, regenerative and non-regenerative processes are associated with distinct qualitative change in MSCs heterogeneity that determine the repair final outcomes.2026-03-16T00:00:00Z2026-03-16T12:51:02.929Z2024-10-22T14:27:15.228ZE-MTAB-14552ERP165382EFO_0002944EFO_0004170EFO_0005684EFO_0005518EFO_0004184