biostudies-arrayexpressSandhya Visweswariah SMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14556A knock-in mouse model has been generated where the serine residue at position 839 in the catalytic domain of receptor guanylyl cyclase C (product of the Gucy2c gene) has been converted to an isoleucine residue. Organoids were prepared from the small intestine of wild-type C57BL6N Tac mice and the mutant S839I mouse by established procedures. RNA was prepared from organoids and subjected to RNAseq analysis.biostudies-arrayexpressSample Collection - Small intestine was isolated from mice for organoid preparation. Preparation was as per protocols published by StemCellSequencing - Illumina NovaSeq used for sequencing with a minimum of 20 million reads with polyA selectionLibrary Construction - mRNA fragment and synthesis of cDNA followed by end repair and dA tailing. cDNA purified and amplified by PCRSample Treatment - Organoids prepared from wild type and S839I mice were cultured for 3 days after passaging and then treated with interferon gamma or uroguanylin or both together for 16h.Nucleic Acid Extraction - Total RNA purified form cultured organoids using Invitrogen RNA purification kits. RNA was treated with DNAse on the column.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Reads were trimmed, mapped on to the mouse genome and differential gene expression calculated by deepseq2.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq XRNA-seq of coding RNAMus musculusSandhya Visweswariah SfalseRNAseq of small intestinal organoids prepared from wild-type and mutant mice that harbour a point mutation that converts a serine residue at position 839 to an isoleucineA knock-in mouse model has been generated where the serine residue at position 839 in the catalytic domain of receptor guanylyl cyclase C (product of the Gucy2c gene) has been converted to an isoleucine residue. Organoids were prepared from the small intestine of wild-type C57BL6N Tac mice and the mutant S839I mouse by established procedures. RNA was prepared from organoids and subjected to RNAseq analysis.2025-10-10T00:00:00Z2026-06-12T13:45:29.31Z2024-10-25T09:14:57.037ZE-MTAB-14556ERP165484EFO_0002944EFO_0004170EFO_0005518EFO_0003816EFO_0004184EFO_0003969