{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Patrick Nylund"],"study_type":["methylation profiling by array"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14582"],"description":["Epigenetic therapies of the multiple myeloma cell line INA-6 under different treatment conditions"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Growth Protocol - The MM cell line INA-6 was cultured in RPMI-1640 AQmediaTM media (Sigma) supplemented with 10% heat-inactivated FBS (Sigma), 1% GlutaMAXTM-I and antibiotics (penicillin 100U/ml and streptomycin 50 mg/mL; Sigma) and Interleukin-6 (IL-6) at 37°C in a humidified 5% CO2 in-air atmosphere incubator. Exponentially growing cells were seeded at 100.000 cells/mL and incubated overnight before addition of reagents. Every 72h, cells were split in equal numbers and re-plated.","Scaning - Illumina Infinium MethylationEPIC array","Sample Treatment - Exponentially growing INA-6 cells were seeded at 100.000 cells/mL and incubated overnight before addition of vehicle DMSO, 0.5µM UNC1999, 50nM 5-azacytidine or the combination of 0.5µM UNC1999 and 50nM 5-azacytidine for 9 days.","Sample Collection - The INA-6 MM cell line was treated with vehicle DMSO, 0.5µM UNC1999, 50nM 5-azacytidine or the combination of both 0.5µM UNC1999 and 50nM 5-azacytidine for the total of 9 days, and global DNA methylation was assessed after bisulfite conversion.","Nucleic Acid Extraction - Bisulfite conversion was performed using the EZ DNA MethylationTM Kit (Zymo Research #D5004) with 250 ng of DNA per sample. The bisulfite-converted DNA was eluted in 15μl according to the manufacturer´s protocol.","Labeling - The ddCTP and ddGTP are labeled with biotin while ddATP and ddUTP are labeled DNP.","Hybridization - Hybridization is followed by single-base extension with hapten-labeled dideoxynucleotides."],"figure_sub":["MIAME Score","Raw Data","Organization","Assays and Data","MAGE-TAB Files","Array Designs"],"pubmed_authors":["Antonia Kalushkova","Patrick Nylund"],"additional_accession":[]},"is_claimable":false,"name":"EPIC INA6","description":"Epigenetic therapies of the multiple myeloma cell line INA-6 under different treatment conditions","dates":{"release":"2025-12-30T00:00:00Z","modification":"2025-12-30T02:02:06.681Z","creation":"2024-11-03T23:03:01.126Z"},"accession":"E-MTAB-14582","cross_references":{"EFO":["EFO_0002944","EFO_0003814","EFO_0003813","EFO_0003789","EFO_0002759","EFO_0005518","EFO_0003815","EFO_0003969"]}}