biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsPatrick Nylundmethylation profiling by arrayHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14582Epigenetic therapies of the multiple myeloma cell line INA-6 under different treatment conditionsbiostudies-arrayexpressGrowth Protocol - The MM cell line INA-6 was cultured in RPMI-1640 AQmediaTM media (Sigma) supplemented with 10% heat-inactivated FBS (Sigma), 1% GlutaMAXTM-I and antibiotics (penicillin 100U/ml and streptomycin 50 mg/mL; Sigma) and Interleukin-6 (IL-6) at 37°C in a humidified 5% CO2 in-air atmosphere incubator. Exponentially growing cells were seeded at 100.000 cells/mL and incubated overnight before addition of reagents. Every 72h, cells were split in equal numbers and re-plated.Scaning - Illumina Infinium MethylationEPIC arraySample Treatment - Exponentially growing INA-6 cells were seeded at 100.000 cells/mL and incubated overnight before addition of vehicle DMSO, 0.5µM UNC1999, 50nM 5-azacytidine or the combination of 0.5µM UNC1999 and 50nM 5-azacytidine for 9 days.Sample Collection - The INA-6 MM cell line was treated with vehicle DMSO, 0.5µM UNC1999, 50nM 5-azacytidine or the combination of both 0.5µM UNC1999 and 50nM 5-azacytidine for the total of 9 days, and global DNA methylation was assessed after bisulfite conversion.Nucleic Acid Extraction - Bisulfite conversion was performed using the EZ DNA MethylationTM Kit (Zymo Research #D5004) with 250 ng of DNA per sample. The bisulfite-converted DNA was eluted in 15μl according to the manufacturer´s protocol.Labeling - The ddCTP and ddGTP are labeled with biotin while ddATP and ddUTP are labeled DNP.Hybridization - Hybridization is followed by single-base extension with hapten-labeled dideoxynucleotides.MIAME ScoreRaw DataOrganizationAssays and DataMAGE-TAB FilesArray DesignsAntonia KalushkovaPatrick NylundfalseEPIC INA6Epigenetic therapies of the multiple myeloma cell line INA-6 under different treatment conditions2025-12-30T00:00:00Z2025-12-30T02:02:06.681Z2024-11-03T23:03:01.126ZE-MTAB-14582EFO_0002944EFO_0003814EFO_0003813EFO_0003789EFO_0002759EFO_0005518EFO_0003815EFO_0003969