biostudies-arrayexpressLara BuermannHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14585The clinical utility of T-cell agonistic antibodies in cancer therapy, much less their ability to stimulate intratumoral T-cells in patients, has eluded us. T-cell agonistic antibodies such as anti-CD27 can induce clinically meaningful anti-tumor activity. The combination of anti-CD27 agonist varlilumab and tumor-depleting anti-CD20 rituximab was investigated in patients with relapsed/refractory B-cell non-Hodgkin lymphoma (B-NHL) (RiVa trial;NCT03307746). Our experiments detail scRNA-seq data from 6 B-cell NHL patients, including both pre and on-treatment biopsies taken from lymph nodes.biostudies-arrayexpressSequencing - Samples were then sequenced on an Illumina NovaSeq 6000 sequencer by the OGC with a read configuration of 91 bp paired-end.Nucleic Acid Extraction - scRNAseq of pre- and on-treatment biopsies were performed using the 10X Genomics (Pleasanton, California) Chromium Single Cell 3’ Library & Gel Bead Kit (chemistry version 3.1) as per protocol, from thawed single-cell suspensions.Sample Collection - Fresh cores of tumors cores collected in RPMI were minced into 1 mm3 pieces or smaller using a scalpel and added to Liberase™ DL (Roche, Basel) RPMI for 15 minutes at 37 oC on an orbital shaker at 250 rpm. The sample mixture was then passed through a 70 µm filter, washed four times, red cell lysis (Qiagen) undertaken, and washed twice more. Resultant single cell suspension was cryopreserved in 50% v/v human AB serum (Sigma, Burlington), 40% supplemented RPMI and 10% v/v DMSO (Sigma, Burlington).Library Construction - scRNAseq of pre- and on-treatment biopsies were performed using the 10X Genomics (Pleasanton, California) Chromium Single Cell 3’ Library & Gel Bead Kit (chemistry version 3.1) as per protocol, from thawed single-cell suspensions.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Processed data are of filtered counts produced by cellranger, not from further pre-processing and filtering in standard analysis workflows.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNA from single cellsHomo sapiensSean LimLara BuermannfalseAnti-CD27 immunotherapy induces intratumoral immunostimulation in B-cell lymphoma: the multi centre RiVa trial (scRNA-Seq Data for RiVa)The clinical utility of T-cell agonistic antibodies in cancer therapy, much less their ability to stimulate intratumoral T-cells in patients, has eluded us. T-cell agonistic antibodies such as anti-CD27 can induce clinically meaningful anti-tumor activity. The combination of anti-CD27 agonist varlilumab and tumor-depleting anti-CD20 rituximab was investigated in patients with relapsed/refractory B-cell non-Hodgkin lymphoma (B-NHL) (RiVa trial;NCT03307746). Our experiments detail scRNA-seq data from 6 B-cell NHL patients, including both pre and on-treatment biopsies taken from lymph nodes.2025-08-09T00:00:00Z2025-08-10T00:01:06.453Z2024-11-06T17:24:21.852ZE-MTAB-14585ERP165978EFO_0002944EFO_0004170EFO_0005684EFO_0005518EFO_0003816EFO_0004184