biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsDr. Santosh ChauhanIllumina HiSeq 4000RNA-seq of coding RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14607To undersatnd the molecular mechanism by which human DZIP3 controls innate antiviral immune signalingbiostudies-arrayexpressSequencing - The library was sequenced using the HiSeq 4000 Illumina platform.Library Construction - After assessing the quality of RNA, 800-900 ng of total RNA was subjected to NEBNext® ultra™ directional RNA library prep kit for Illumina® (#E7420) using NEBNextPoly(A) mRNA magnetic isolation module (NEB #E7490). The QC of the prepared library was performed using High-Sensitivity Tape Station Kit (Agilent 2200, 5067-5585 (reagents) #5067-5584 ( screen tapes)) for fragment length distribution and Qubit dsDNA HS assay kit (Invitrogen, Q32851) for quantifying the library.Nucleic Acid Extraction - RNA was extracted from THP-1 cells(three biological replicates) using RNeasy mini kit (QIAGEN, #74104). The quality and quantity of total RNA was checked using agarose gel and Qubit 4.0.Sample Collection - THP-1 control and DZIP3 KD cells were collected from 3 different wells for each group in a 6 well plateOrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesDr. Santosh ChauhanfalseRNA-seq of Control and DZIP3 siRNA Knockdown THP-1 cellsTo undersatnd the molecular mechanism by which human DZIP3 controls innate antiviral immune signaling2025-11-07T00:00:00Z2025-11-07T02:02:18.035Z2024-11-15T11:40:09.864ZE-MTAB-14607ERP166273E-MTAB-14608EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184