{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Dr. Santosh Chauhan"],"instrument_platform":["Illumina HiSeq 4000"],"study_type":["RNA-seq of coding RNA"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14608"],"description":["To undersatnd the molecular mechanism by which human DZIP3 controls innate antiviral immune signaling"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - The library was sequenced using the HiSeq 4000 Illumina platform.","Library Construction - After assessing the quality of RNA, 800-900 ng of total RNA was subjected to NEBNext® ultra™ directional RNA library prep kit for Illumina® (#E7420) using NEBNextPoly(A) mRNA magnetic isolation module (NEB #E7490). The QC of the prepared library was performed using High-Sensitivity Tape Station Kit (Agilent 2200, 5067-5585 (reagents) #5067-5584 ( screen tapes)) for fragment length distribution and Qubit dsDNA HS assay kit (Invitrogen, Q32851) for quantifying the library.","Sample Collection - HT-29 control and DZIP3 KD cells were collected from 3 different wells for each group in a 6 well plate","Nucleic Acid Extraction - RNA was extracted from HT-29 cells(three biological replicates) using RNeasy mini kit (QIAGEN, #74104). The quality and quantity of total RNA was checked using agarose gel and Qubit 4.0."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Dr. Santosh Chauhan"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of Control and DZIP3 shRNA Knockdown HT-29 cells","description":"To undersatnd the molecular mechanism by which human DZIP3 controls innate antiviral immune signaling","dates":{"release":"2025-11-08T00:00:00Z","modification":"2025-11-08T02:02:19.884Z","creation":"2024-11-15T11:40:29.243Z"},"accession":"E-MTAB-14608","cross_references":{"ENA":["ERP166275"],"Biostudies":["E-MTAB-14607"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}