{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Ramesh Yelagandula"],"instrument_platform":["Illumina NovaSeq X"],"study_type":["ATAC-seq"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14658"],"description":["Analyzing chromatin accessibility in MEF cells pre treatment and post treatment of LLOMe at different time points. (30mins, 3hrs and 30hrs)."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - Untreated and treated MEFs (50,000 cells) are collected in duplicates from different plates of each group and subjected to Tagmentation using Tn5 (Zymo-seq ATAC Library kit: D5458)","Nucleic Acid Extraction - The Tagmented DNA was isolated using the DNA purification columns provided in the Zymo kit.","Sequencing - The library was sequenced using the Novaseq X illumina platform","Library Construction - The ATACseq library was PCR amplified using the Unique Dual Index Nextera primers. Amplified libraries are purified using ampure beads."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Putchala Ravi Kiran","Ramesh Yelagandula","Santosh Chauhan","Kollori Dhar"],"additional_accession":[]},"is_claimable":false,"name":"ATAC sequencing of untreated and LLOMe treated MEF cells","description":"Analyzing chromatin accessibility in MEF cells pre treatment and post treatment of LLOMe at different time points. (30mins, 3hrs and 30hrs).","dates":{"release":"2025-07-04T00:00:00Z","modification":"2025-07-04T09:00:46.599Z","creation":"2024-12-02T21:53:26.575Z"},"accession":"E-MTAB-14658","cross_references":{"ENA":["ERP166717"],"EFO":["EFO_0002944","EFO_0007045","EFO_0004170","EFO_0005518","EFO_0004184"]}}