biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsRamesh YelagandulaIllumina NovaSeq XATAC-seqMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14658Analyzing chromatin accessibility in MEF cells pre treatment and post treatment of LLOMe at different time points. (30mins, 3hrs and 30hrs).biostudies-arrayexpressSample Collection - Untreated and treated MEFs (50,000 cells) are collected in duplicates from different plates of each group and subjected to Tagmentation using Tn5 (Zymo-seq ATAC Library kit: D5458)Nucleic Acid Extraction - The Tagmented DNA was isolated using the DNA purification columns provided in the Zymo kit.Sequencing - The library was sequenced using the Novaseq X illumina platformLibrary Construction - The ATACseq library was PCR amplified using the Unique Dual Index Nextera primers. Amplified libraries are purified using ampure beads.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesPutchala Ravi KiranRamesh YelagandulaSantosh ChauhanKollori DharfalseATAC sequencing of untreated and LLOMe treated MEF cellsAnalyzing chromatin accessibility in MEF cells pre treatment and post treatment of LLOMe at different time points. (30mins, 3hrs and 30hrs).2025-07-04T00:00:00Z2025-07-04T09:00:46.599Z2024-12-02T21:53:26.575ZE-MTAB-14658ERP166717EFO_0002944EFO_0007045EFO_0004170EFO_0005518EFO_0004184