biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsYohei SatoIllumina NovaSeq 6000RNA-seq of coding RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14707Human FOXP3+ regulatory T cells were isolated as CD4+CD25+CD127- population from PBMCs. Cells were stimulated with TCR stimulation with the presence of rhIL-2.biostudies-arrayexpressSample Collection - Human Tregs were isolated by cell sorting.Library Construction - Library was generated with NEBNext UltraTM II Directional RNA Library Prep Kit.Sequencing - Libraries were sequenced by Novaseq 6000.Nucleic Acid Extraction - RNA were extracted from sorted cells by RNeasy micro kit.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesYohei SatofalseRNA-seq on human regulaotry T cells before and after TCR stimulationHuman FOXP3+ regulatory T cells were isolated as CD4+CD25+CD127- population from PBMCs. Cells were stimulated with TCR stimulation with the presence of rhIL-2.2025-06-01T00:00:00Z2024-12-17T14:26:31.078Z2024-12-17T14:26:31.078ZE-MTAB-14707ERP167244EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184