{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Nora Doleschall"],"organism":["Homo sapiens"],"software":["Agilent Feature Extraction"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14716"],"description":["This experiment was used to assess levels of intra- and extracellular/secreted miRNAs in a primary HUVEC culture over time."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - Conditioned media was removed from cell cultures, and cleared of debris by centrifugation at 5000g. Cells were then washed with HBSS buffer then TRI reagent was added to each well. Cell lysates and conditioned media were stored at -80 until RNA extraction.","Hybridization - Hybridisation was performed over 20 hours at 55 degrees C, rotating 20rpm in Agilent hybridisation oven","Growth Protocol - Cells were grown for 15-240 minutes in low-serum conditions in MCDB medium.","Labeling - Cy3-pCp labelling was performed according to Agilent recommendations","Scaning - Agilent Microarray Scanner (G2505C) was used for array scanning and Agilent Feature Extraction software used for image processing.","Nucleic Acid Extraction - smallRNA enriched RNA extraction was performed using Qiagen miRNeasy kit"],"figure_sub":["MIAME Score","Raw Data","Organization","Assays and Data","Processed Data","MAGE-TAB Files","Array Designs"],"data_protocol":["Data Transformation - Agilent Feature Extraction software was used to assign probes to genes and to perform background correction and error evaluation for individual probes"],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Agilent Microarray Scanner G2505C","Agilent Hybridisation Oven G2545A"],"study_type":["microRNA profiling by array"],"species":["Homo sapiens"],"pubmed_authors":["László Cervenak","Nora Doleschall"],"additional_accession":[]},"is_claimable":false,"name":"Dynamics of intra- and extracellular miRNA expression changes over time in culture","description":"This experiment was used to assess levels of intra- and extracellular/secreted miRNAs in a primary HUVEC culture over time.","dates":{"release":"2025-06-30T00:00:00Z","modification":"2024-12-19T21:36:45.463Z","creation":"2024-12-19T21:36:45.463Z"},"accession":"E-MTAB-14716","cross_references":{"Biostudies":["E-MTAB-14672"],"EFO":["EFO_0002944","EFO_0003814","EFO_0000753","EFO_0003813","EFO_0003789","EFO_0005518","EFO_0003816","EFO_0003815"]}}