{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Nora Doleschall"],"organism":["Homo sapiens"],"software":["Agilent Feature Extraction","None"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14718"],"description":["This experiment was used to compare the miRNA expression profiles of paired healthy blood serum, blood plasma, blood-derived cellular components and their conditioned media."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Growth Protocol - Blood cellular components were collected from EDTA-anticoagulated whole blood after removing the plasma by centrifugation. These were washed twice with PBS then incubated in RPMI-1640 without FBS for 6 hours. WBCs as well as conditioned media were stored at – 80°C until RNA extraction.","Hybridization - Hybridisation was performed over 20 hours at 55 degrees C, rotating 20rpm in Agilent hybridisation oven","Sample Collection - Blood samples were taken via venepuncture, and were immediately transferred to the processing laboratory, where the cells and the supernatant – serum and EDTA-anticoagulated plasma – were separated by centrifugation. Serum and plasma aliquots were prepared, immediately frozen and stored at − 80 °C until the measurements were performed.","Labeling - Cy3-pCp labelling was performed according to Agilent recommendations","Scaning - Agilent Microarray Scanner (G2505C) was used for array scanning and Agilent Feature Extraction software used for image processing.","Nucleic Acid Extraction - smallRNA enriched RNA extraction was performed using Qiagen miRNeasy kit"],"figure_sub":["MIAME Score","Raw Data","Organization","Assays and Data","Processed Data","MAGE-TAB Files","Array Designs"],"data_protocol":["Data Transformation - Agilent Feature Extraction software was used to assign probes to genes and to perform background correction and error evaluation for individual probes"],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Agilent Microarray Scanner G2505C","Tabletop microfuge, Nanodrop","PC","Agilent Hybridisation Oven G2545A","Tabletop centrifuge","None"],"study_type":["microRNA profiling by array"],"species":["Homo sapiens"],"pubmed_authors":["László Cervenak","Nora Doleschall"],"additional_accession":[]},"is_claimable":false,"name":"miRNA microarray experiment elucidating the contribution of intra-and extracellular leukocyte-derived miRNAs to paired blood serum and plasma samples","description":"This experiment was used to compare the miRNA expression profiles of paired healthy blood serum, blood plasma, blood-derived cellular components and their conditioned media.","dates":{"release":"2025-06-30T00:00:00Z","modification":"2024-12-19T22:13:46.341Z","creation":"2024-12-19T22:13:46.341Z"},"accession":"E-MTAB-14718","cross_references":{"Biostudies":["E-MTAB-14717","E-MTAB-14672"],"EFO":["EFO_0002944","EFO_0003814","EFO_0000753","EFO_0003813","EFO_0003789","EFO_0005518","EFO_0003816","EFO_0003815"]}}