biostudies-arrayexpressNora DoleschallHomo sapiensAgilent Feature ExtractionNonehttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14718This experiment was used to compare the miRNA expression profiles of paired healthy blood serum, blood plasma, blood-derived cellular components and their conditioned media.biostudies-arrayexpressGrowth Protocol - Blood cellular components were collected from EDTA-anticoagulated whole blood after removing the plasma by centrifugation. These were washed twice with PBS then incubated in RPMI-1640 without FBS for 6 hours. WBCs as well as conditioned media were stored at – 80°C until RNA extraction.Hybridization - Hybridisation was performed over 20 hours at 55 degrees C, rotating 20rpm in Agilent hybridisation ovenSample Collection - Blood samples were taken via venepuncture, and were immediately transferred to the processing laboratory, where the cells and the supernatant – serum and EDTA-anticoagulated plasma – were separated by centrifugation. Serum and plasma aliquots were prepared, immediately frozen and stored at − 80 °C until the measurements were performed.Labeling - Cy3-pCp labelling was performed according to Agilent recommendationsScaning - Agilent Microarray Scanner (G2505C) was used for array scanning and Agilent Feature Extraction software used for image processing.Nucleic Acid Extraction - smallRNA enriched RNA extraction was performed using Qiagen miRNeasy kitMIAME ScoreRaw DataOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsData Transformation - Agilent Feature Extraction software was used to assign probes to genes and to perform background correction and error evaluation for individual probesUnknownTranscriptomicsGenomicsProteomicsAgilent Microarray Scanner G2505CTabletop microfuge, NanodropPCAgilent Hybridisation Oven G2545ATabletop centrifugeNonemicroRNA profiling by arrayHomo sapiensLászló CervenakNora DoleschallfalsemiRNA microarray experiment elucidating the contribution of intra-and extracellular leukocyte-derived miRNAs to paired blood serum and plasma samplesThis experiment was used to compare the miRNA expression profiles of paired healthy blood serum, blood plasma, blood-derived cellular components and their conditioned media.2025-06-30T00:00:00Z2024-12-19T22:13:46.341Z2024-12-19T22:13:46.341ZE-MTAB-14718E-MTAB-14717E-MTAB-14672EFO_0002944EFO_0003814EFO_0000753EFO_0003813EFO_0003789EFO_0005518EFO_0003816EFO_0003815