{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Tao Zhou"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14741"],"description":["RNA-Seq analyses were performed on OVCAR3 cells transfected with si-NC (control), si-LINC00654, and si-HuR, to study the interactons between LINC00654 and HuR."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - Total RNA was extracted from cells and tissues using the RNA isolator Total RNA Extraction Reagent (Vazyme, Nanjing, China) according to the manufacturer's instructions.","Library Construction - RNA was then reverse-transcribed into cDNA using the HiScript III RT SuperMix qPCR kit (Vazyme).","Sample Collection - OVCAR3 cells were purchased from the Institute of Biochemistry and Cell Biology of the Chinese Academy of Sciences (Shanghai, China). OVCAR3 cells were supplemented with 20% fetal bovine serum. Cells were harvested 48 hours post-transfection for subsequent experiments, including transfection efficiency and functional assays.","Sequencing - RNA was extracted from OVCAR3 cells transfected with si-LINC00654, si-HuR, or si-NC (n = 3 per group) and sequenced with the 2 × 150 bp paired-end (PE150) sequencing strategy on an Illumina NovaSeq™ 6000 platform according to the vendor's protocol."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - Transcript quantification was achieved with featureCounts, and differential expression analysis was carried out using DESeq2.Differentially expressed transcripts (DETs) were defined with fold change > 2 and false discovery rate (FDR) < 0.05."],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of total RNA"],"species":["Homo sapiens"],"pubmed_authors":["Tao Zhou"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptomic analysis of the interactions between LINC00654 and HuR","description":"RNA-Seq analyses were performed on OVCAR3 cells transfected with si-NC (control), si-LINC00654, and si-HuR, to study the interactons between LINC00654 and HuR.","dates":{"release":"2025-12-31T00:00:00Z","modification":"2025-12-31T02:02:15.331Z","creation":"2025-01-02T16:19:16.652Z"},"accession":"E-MTAB-14741","cross_references":{"ENA":["ERP167462"],"EFO":["EFO_0002944","EFO_0004170","EFO_0009653","EFO_0005518","EFO_0003816","EFO_0004184"]}}