biostudies-arrayexpressTao ZhouHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14741RNA-Seq analyses were performed on OVCAR3 cells transfected with si-NC (control), si-LINC00654, and si-HuR, to study the interactons between LINC00654 and HuR.biostudies-arrayexpressNucleic Acid Extraction - Total RNA was extracted from cells and tissues using the RNA isolator Total RNA Extraction Reagent (Vazyme, Nanjing, China) according to the manufacturer's instructions.Library Construction - RNA was then reverse-transcribed into cDNA using the HiScript III RT SuperMix qPCR kit (Vazyme).Sample Collection - OVCAR3 cells were purchased from the Institute of Biochemistry and Cell Biology of the Chinese Academy of Sciences (Shanghai, China). OVCAR3 cells were supplemented with 20% fetal bovine serum. Cells were harvested 48 hours post-transfection for subsequent experiments, including transfection efficiency and functional assays.Sequencing - RNA was extracted from OVCAR3 cells transfected with si-LINC00654, si-HuR, or si-NC (n = 3 per group) and sequenced with the 2 × 150 bp paired-end (PE150) sequencing strategy on an Illumina NovaSeq™ 6000 platform according to the vendor's protocol.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Transcript quantification was achieved with featureCounts, and differential expression analysis was carried out using DESeq2.Differentially expressed transcripts (DETs) were defined with fold change > 2 and false discovery rate (FDR) < 0.05.UnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of total RNAHomo sapiensTao ZhoufalseTranscriptomic analysis of the interactions between LINC00654 and HuRRNA-Seq analyses were performed on OVCAR3 cells transfected with si-NC (control), si-LINC00654, and si-HuR, to study the interactons between LINC00654 and HuR.2025-12-31T00:00:00Z2025-12-31T02:02:15.331Z2025-01-02T16:19:16.652ZE-MTAB-14741ERP167462EFO_0002944EFO_0004170EFO_0009653EFO_0005518EFO_0003816EFO_0004184