biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsKian O'NeilSurgical ToolsIllumina NextSeq P2RNeasy KitNextSeq 2000RNA-seq of total RNAMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14742The intent of this experiment was to identify changes in gene expression of UUO kidneys treated with a novel ACLY inhibitor.biostudies-arrayexpressSample Collection - Mouse kidney cortices were isolated, stored in liquid nitrogen, and processed for RNA extraction. Tissue samples were collected with care to preserve RNA integrity, ensuring high-quality downstream analysis.Library Construction - Poly-A RNA was enriched from total RNA to select for mRNA transcripts. Library preparation, including cDNA synthesis and adapter ligation, was performed by the McMaster Genomics Facility using protocols optimized for the Illumina NextSeq P2 sequencing platform.Sequencing - Sequencing was carried out on the Illumina NextSeq P2 platform, designed for mid-throughput RNA sequencing applications. The quality-checked libraries were sequenced to detect differential gene expression with a focus on achieving high read depth and accuracy.Nucleic Acid Extraction - RNA was extracted from mouse kidney cortices using the RNeasy Kit (Qiagen), following the manufacturer’s standard protocol. This method includes steps for cell lysis, removal of contaminants, and RNA elution to obtain high-purity RNA for sequencing.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesKian O'NeilfalseRNAseq of kidney tissue from sham or UUO mice treated with vehicle or EVT0185 (ACLY inhibitor)The intent of this experiment was to identify changes in gene expression of UUO kidneys treated with a novel ACLY inhibitor.2026-01-01T00:00:00Z2026-01-01T02:02:00.953Z2025-01-03T09:19:04.923ZE-MTAB-14742ERP167467EFO_0002944EFO_0004170EFO_0009653EFO_0005518EFO_0004184