biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsChew Weng ChengIllumina HiSeq 1500IlluminaRNA-seq of coding RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14746The metabolite beta-aminoisobutyric acid (BAIBA) as an exercise-induced muscle-derived signal regulating adipose tissue metabolism during exercise. Here, we determine that BAIBA mimics aerobic exercise training effects on human skeletal myocytes.biostudies-arrayexpressSample Collection - Adult human skeletal myoblasts were grown in human skeletal muscle cell growth medium at 37 °C within a humidified atmosphere at 5% CO2. Subculture of human skeletal myoblasts occurred once 85–95% confluency was reached. Experiments were limited to the 5th passage. Human skeletal myoblasts were seeded at 9500 cells per cm2. Once confluent, myoblasts were cultured for 6–8 days in skeletal muscle differentiation media to induce myoblast differentiation to myotubes. Myoblasts were treated with either human skeletal muscle cell growth medium containing BAIBA (10 μM) immediately after plating to assess proliferation or human skeletal muscle cell differentiation medium containing BAIBA (10 µM) once confluent to assess effects of BAIBA on differentiation.Nucleic Acid Extraction - Total RNA was prepared from tissues using TriZol reagent (Sigma).Library Construction - RNA sequencing was performed by University of Leeds Genomics Facility. First, the mRNA is fragmented randomly by adding fragmentation buffer, then the cDNA is synthesized by using mRNA template and random hexamers primer, after which a custom second-strand synthesis buffer (Illumina) , dNTPs, RNase H and DNA polymerase I are added to initiate the second-strand synthesis. Second, after a series of terminal repair, A ligation and sequencing adaptor ligation, the double-stranded cDNA library is completed through size selection and PCR enrichment.Sequencing - The qualified libraries are fed into Illumina sequencers after pooling according to its effective concentration and expected data volume.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesLee RobertsHelene DaouChew Weng ChengfalseBAIBA in human skeletal myocytesThe metabolite beta-aminoisobutyric acid (BAIBA) as an exercise-induced muscle-derived signal regulating adipose tissue metabolism during exercise. Here, we determine that BAIBA mimics aerobic exercise training effects on human skeletal myocytes.2026-06-22T00:00:00Z2026-06-22T09:00:01.103Z2025-01-10T16:58:34.773ZE-MTAB-14746ERP167709EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184