biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsChew Weng ChengIllumina HiSeq 1500IlluminaRNA-seq of coding RNAMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14747The metabolite beta-aminoisobutyric acid (BAIBA) as an exercise-induced muscle-derived signal regulating adipose tissue metabolism during exercise. Here, we determine that BAIBA mimics aerobic exercise training effects on muscle metabolism, morphology and function to enhance exercise performance in mice.biostudies-arrayexpressNucleic Acid Extraction - Total RNA was prepared from tissues using TriZol reagent (Sigma).Library Construction - RNA sequencing was performed by University of Leeds Genomics Facility. First, the mRNA is fragmented randomly by adding fragmentation buffer, then the cDNA is synthesized by using mRNA template and random hexamers primer, after which a custom second-strand synthesis buffer (Illumina) , dNTPs, RNase H and DNA polymerase I are added to initiate the second-strand synthesis. Second, after a series of terminal repair, A ligation and sequencing adaptor ligation, the double-stranded cDNA library is completed through size selection and PCR enrichment.Sequencing - The qualified libraries are fed into Illumina sequencers after pooling according to its effective concentration and expected data volume.Sample Collection - Six-week-old male C57BL6/J mice (Charles River, UK) were weight-matched and assigned to groups for treatment. Mice were treated with 100 mg/kg/day BAIBA in their drinking water for 6 weeks and fed standard chow. Mice were killed by cervical dislocation.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesLee RobertsHelene DaouChew Weng ChengfalseBAIBA in soleus from BAIBA-treated HFD-fed miceThe metabolite beta-aminoisobutyric acid (BAIBA) as an exercise-induced muscle-derived signal regulating adipose tissue metabolism during exercise. Here, we determine that BAIBA mimics aerobic exercise training effects on muscle metabolism, morphology and function to enhance exercise performance in mice.2026-06-22T00:00:00Z2026-06-22T09:00:39.166Z2025-01-10T17:17:39.549ZE-MTAB-14747ERP167710EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184