{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Kateřina Večerková"],"instrument_platform":["NextSeq 500"],"study_type":["RNA-seq of coding RNA"],"organism":["Rattus norvegicus"],"species":["Rattus norvegicus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14749"],"description":["This study investigates the molecular mechanisms underlying salt-sensitive hypertension in a model of primary aldosteronism using RNA-Seq analysis of Sprague Dawley rat skin. Unilaterally nephrectomized rats were treated with aldosterone or vehicle and fed a high-salt or low-salt diet. Tissue electrolytes were quantified using atomic absorption spectrometry, and gene expression profiles were analyzed using RNA-Seq. Key findings include a reduction in plasma, carcass, and skin potassium concentrations in aldosterone-treated rats on a high-salt diet before significant increases in mean arterial pressure and systemic vascular resistance. Gene set enrichment analysis (GSEA) revealed altered expression of genes involved in hyperosmotic response and monovalent inorganic anion homeostasis prior to hypertension onset, and in myofibril assembly, skeletal muscle contraction, and histone methylation after hypertension establishment. These data provide insights into the role of tissue electrolyte imbalance and related gene expression changes in the pathogenesis of salt-sensitive hypertension."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - Library preparation was done with KAPA mRNA HyperPrep Kit for Illumina. Library size distribution was evaluated on the Agilent 2100 Bio-analyzer using the High Sensitivity DNA Kit (Agilent).","Sequencing - Libraries were sequenced on the Illumina NextSeq® 500 instrument using 76bp single-end configuration.","Nucleic Acid Extraction - RNA was isolated using the RNeasy Fibrous Tissue Kit (Qiagen) with water elution and stored at -80°C until further processing.","Sample Treatment - Rats weighing approximately 300 g underwent unilateral nephrectomy and were individually housed with free access to tap water and a low-salt diet (AIN-76A diet containing 0.26% NaCl and 0.36% potassium; Diet No. 100000, Dyets Inc., Bethlehem, PA). Four weeks post-surgery, rats were randomized to receive a continuous subcutaneous infusion of either aldosterone or vehicle using subcutaneously implanted osmotic minipumps (2 pumps per rat). Aldosterone was infused at a dose of 1.5 microgram/h with a flow rate of 0.3 microliter/h. Following minipump implantation, rats were maintained on the low-salt diet and tap water for two weeks to recover. Subsequently, all rats were switched to a high-salt diet (AIN-76A diet containing 4% NaCl; Diet No. 113756, Dyets Inc., Bethlehem, PA) with tap water provided ad libitum. The high-salt diet was compositionally identical to the low-salt diet except for its increased salt content.","Sample Collection - Rats were euthanized by cervical dislocation. Carcasses, skin, and plasma were collected from aldosterone- and vehicle-treated rats after 2 and 14 days on a high-salt diet for the measurement of Na⁺, K⁺, and Cl⁻ concentrations. Skin samples for RNA extraction were flash-frozen and homogenized using a Tissuelyser (Qiagen)."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Petr Mlejnek","Michal Pravenec","Kateřina Večerková"],"additional_accession":[]},"is_claimable":false,"name":"RNA-Seq of Sprague Dawley Rat Skin Treated with Aldosterone or Vehicle on High-Salt Diet for 2 or 14 Days","description":"This study investigates the molecular mechanisms underlying salt-sensitive hypertension in a model of primary aldosteronism using RNA-Seq analysis of Sprague Dawley rat skin. Unilaterally nephrectomized rats were treated with aldosterone or vehicle and fed a high-salt or low-salt diet. Tissue electrolytes were quantified using atomic absorption spectrometry, and gene expression profiles were analyzed using RNA-Seq. Key findings include a reduction in plasma, carcass, and skin potassium concentrations in aldosterone-treated rats on a high-salt diet before significant increases in mean arterial pressure and systemic vascular resistance. Gene set enrichment analysis (GSEA) revealed altered expression of genes involved in hyperosmotic response and monovalent inorganic anion homeostasis prior to hypertension onset, and in myofibril assembly, skeletal muscle contraction, and histone methylation after hypertension establishment. These data provide insights into the role of tissue electrolyte imbalance and related gene expression changes in the pathogenesis of salt-sensitive hypertension.","dates":{"release":"2026-01-20T00:00:00Z","modification":"2026-01-20T02:02:13.66Z","creation":"2025-01-14T12:57:12.006Z"},"accession":"E-MTAB-14749","cross_references":{"ENA":["ERP167855"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184","EFO_0003969"]}}