{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":[null],"study_type":["transcription profiling by array"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14750"],"description":["This repository provides the Affymetrix microarray data (*.CEL files) generated for our study on EYS-mutant (mEYS) vs. control iPSC-derived retinal pigment epithelium (RPE). The dataset is part of a larger project that explores how CRISPR/Cas9 correction of EYS mutations influences photoreceptor and RPE development. Below is a concise summary of the microarray protocol from our manuscript’s Materials & Methods."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - High Pure RNA Tissue Kit (or similar) o Quality Control: RNA integrity assessed via Bioanalyzer (Agilent), with RNA Integrity Numbers (RIN) > 8.80 selected for downstream analysis.","Hybridization - 2.3 µg of labeled cDNA was applied to the microarray chips and incubated per the manufacturer’s protocol.","Scaning - GeneChip® Scanner 3000 7G (Affymetrix) o Software: GeneChip® Command Console Software 2.0 for initial intensity data (.CEL file) generation.","Labeling - 100 ng of total RNA per sample converted to cDNA, followed by end-labeling with biotin to create ssDNA probes.","Sample Collection - Cell collected in cell lysis buffer (e.g. RLT buffer)"],"figure_sub":["MIAME Score","Raw Data","Assays and Data","Processed Data","organisation","MAGE-TAB Files","Array Designs"],"pubmed_authors":["Álvaro Plaza Reyes","Francisco Diaz Corrales"],"data_protocol":["Data Transformation - SST-RMA (Signal Space Transformation–Robust Microarray Analysis) applied to generate gene-level expression metrics."],"additional_accession":[]},"is_claimable":false,"name":"Transcriptomic Profiles of EYS-Mutant and Control iPSC-RPE (Affymetrix Data)","description":"This repository provides the Affymetrix microarray data (*.CEL files) generated for our study on EYS-mutant (mEYS) vs. control iPSC-derived retinal pigment epithelium (RPE). The dataset is part of a larger project that explores how CRISPR/Cas9 correction of EYS mutations influences photoreceptor and RPE development. Below is a concise summary of the microarray protocol from our manuscript’s Materials & Methods.","dates":{"release":"2025-06-29T00:00:00Z","modification":"2025-03-24T10:44:16.118Z","creation":"2025-01-14T12:57:42.257Z"},"accession":"E-MTAB-14750","cross_references":{"EFO":["EFO_0002768","EFO_0002944","EFO_0003814","EFO_0003813","EFO_0005518","EFO_0003816","EFO_0003815"]}}