biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicstranscription profiling by arrayHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14750This repository provides the Affymetrix microarray data (*.CEL files) generated for our study on EYS-mutant (mEYS) vs. control iPSC-derived retinal pigment epithelium (RPE). The dataset is part of a larger project that explores how CRISPR/Cas9 correction of EYS mutations influences photoreceptor and RPE development. Below is a concise summary of the microarray protocol from our manuscript’s Materials & Methods.biostudies-arrayexpressNucleic Acid Extraction - High Pure RNA Tissue Kit (or similar) o Quality Control: RNA integrity assessed via Bioanalyzer (Agilent), with RNA Integrity Numbers (RIN) > 8.80 selected for downstream analysis.Hybridization - 2.3 µg of labeled cDNA was applied to the microarray chips and incubated per the manufacturer’s protocol.Scaning - GeneChip® Scanner 3000 7G (Affymetrix) o Software: GeneChip® Command Console Software 2.0 for initial intensity data (.CEL file) generation.Labeling - 100 ng of total RNA per sample converted to cDNA, followed by end-labeling with biotin to create ssDNA probes.Sample Collection - Cell collected in cell lysis buffer (e.g. RLT buffer)MIAME ScoreRaw DataAssays and DataProcessed DataorganisationMAGE-TAB FilesArray DesignsÁlvaro Plaza ReyesFrancisco Diaz CorralesData Transformation - SST-RMA (Signal Space Transformation–Robust Microarray Analysis) applied to generate gene-level expression metrics.falseTranscriptomic Profiles of EYS-Mutant and Control iPSC-RPE (Affymetrix Data)This repository provides the Affymetrix microarray data (*.CEL files) generated for our study on EYS-mutant (mEYS) vs. control iPSC-derived retinal pigment epithelium (RPE). The dataset is part of a larger project that explores how CRISPR/Cas9 correction of EYS mutations influences photoreceptor and RPE development. Below is a concise summary of the microarray protocol from our manuscript’s Materials & Methods.2025-06-29T00:00:00Z2025-03-24T10:44:16.118Z2025-01-14T12:57:42.257ZE-MTAB-14750EFO_0002768EFO_0002944EFO_0003814EFO_0003813EFO_0005518EFO_0003816EFO_0003815