biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsSeongjun KimIllumina NovaSeq 6000RNA-seq of coding RNASus scrofaSus scrofahttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14775This study utilized RNA-seq data to comprehensively analyze the transcriptome across five tissues (heart, liver, kidney, back fat, and loin) of minipigs. As the demand for organ transplantation continues to rise, xenotransplantation has gained significant attention as a promising solution to address the critical shortage of organ donors. Minipigs are considered a highly promising animal model for xenotransplantation research due to their remarkable physiological and anatomical similarities to humans. To evaluate the potential of minipigs, this study identified tissue-specific genes and differentially expressed genes (DEGs) related to body size, offering an in-depth exploration of their transcriptomic characteristics. The findings revealed that tissue-specific genes such as MYH7B in the heart and ITIH4 in the liver play pivotal roles in physiological and pathological processes. Additionally, DEGs associated with body size, such as DES in the heart and NRAP in the loin, provided valuable insights into the relationship between organ size and functionality. Notably, the expression patterns of immune-related genes such as IFI30, NCK2, NFKB1, and SOS2 demonstrated that minipigs exhibit a greater similarity to humans than to conventional pigs, highlighting their potential as an indispensable model for xenotransplantation and biomedical research.biostudies-arrayexpressSample Collection - To identify tissue-specific body size-related genes, approximately 10 samples representing 5 different tissues were collected from MiniPig individuals with varying body sizes. This comprehensive sampling aimed to capture the relationship between tissue expression patterns and body size variations. All experimental procedures were thoroughly reviewed and approved by the National Institute of Animal Science (NIAS) under approval number NIAS20181295.Nucleic Acid Extraction - Total RNA was extracted from tissue samples using the QIAzol® Lysis Reagent (Qiagen, Cat. No. 79306) and the RNeasy® Mini Kit (Qiagen, Cat. No. 74106) following the manufacturer’s protocol. RNA concentration and purity were assessed using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, USA). Only samples with sufficient quality were used for downstream applications.Library Construction - Individual libraries were generated using Illumina TruSeq™ RNA Sample Preparation Kit (Illumina, San Diego, CA, USA).Sequencing - The sequencing was performed on an Illumina NovaSeq 6000 sequencer.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesSeongjun KimfalseMulti-Tissue RNA-Seq Analysis in Mini Pigs: Body Size and Tissue-Specific ComparisonsThis study utilized RNA-seq data to comprehensively analyze the transcriptome across five tissues (heart, liver, kidney, back fat, and loin) of minipigs. As the demand for organ transplantation continues to rise, xenotransplantation has gained significant attention as a promising solution to address the critical shortage of organ donors. Minipigs are considered a highly promising animal model for xenotransplantation research due to their remarkable physiological and anatomical similarities to humans. To evaluate the potential of minipigs, this study identified tissue-specific genes and differentially expressed genes (DEGs) related to body size, offering an in-depth exploration of their transcriptomic characteristics. The findings revealed that tissue-specific genes such as MYH7B in the heart and ITIH4 in the liver play pivotal roles in physiological and pathological processes. Additionally, DEGs associated with body size, such as DES in the heart and NRAP in the loin, provided valuable insights into the relationship between organ size and functionality. Notably, the expression patterns of immune-related genes such as IFI30, NCK2, NFKB1, and SOS2 demonstrated that minipigs exhibit a greater similarity to humans than to conventional pigs, highlighting their potential as an indispensable model for xenotransplantation and biomedical research.2025-09-11T00:00:00Z2025-09-11T07:49:01.811Z2025-01-24T17:35:54.051ZE-MTAB-14775ERP168643E-MTAB-14776EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184