biostudies-arrayexpressShinkichi IshikawaHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14782Human Pulmonary Alveolar Epithelial Cells (HPAEpiCs) purchased from ScienCell Research Laboratories were cultured at the air-liquid interface (ALI) for 28 days with medium containing Y-27632, A-83-01, and CHIR99021. The gene expression profile of HPAEpiCs was investigated on ALI day 28 by Fixed RNA Profiling.biostudies-arrayexpressSample Collection - HPAEpiCs on ALI day 28 were washed with PBS, incubated in 0.25% trypsin-EDTA, detached from the inserts, and separated by pipetting. Trypsinization was stopped by adding Dulbecco’s Modified Eagle’s Medium supplemented with 10% fetal bovine serum. The cell suspension was then centrifuged, washed, and passed through a 40 µm Flowmi cell strainer (Bel-Art Products, Wayne, NJ, USA). The obtained cells were fixed with 4% formaldehyde in PBS, and subsequently treated with the Quenching Buffer and Enhancer contained in a Chromium Next GEM Single Cell Fixed RNA Sample Preparation Kit (10x Genomics).Library Construction - Isolated single cells were loaded onto a 10x Chromium X (10x Genomics), barcoded, and pre-amplified to construct the sequencing library.Sequencing - The library was sequenced on a DNBSEQ-G400 platform (MGI Tech).Nucleic Acid Extraction - Cell barcoding, amplification, and library construction were performed using a Chromium Fixed RNA Profiling Reagent Kit (10x Genomics) in accordance with the manufacturer's protocol.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - The sequenced reads were subjected to demultiplexing, alignment, barcode counting, UMI counting, and filtering using Cell Ranger (version 7.1.0; 10x Genomics). The human genome (GRCh38-2020-A) was used as a reference to align the reads.UnknownTranscriptomicsGenomicsProteomicsDNBSEQ-G400RNA-seq of coding RNA from single cellsHomo sapiensShinkichi IshikawafalseFixed RNA Profiling of HPAEpiCs Cultured at the ALIHuman Pulmonary Alveolar Epithelial Cells (HPAEpiCs) purchased from ScienCell Research Laboratories were cultured at the air-liquid interface (ALI) for 28 days with medium containing Y-27632, A-83-01, and CHIR99021. The gene expression profile of HPAEpiCs was investigated on ALI day 28 by Fixed RNA Profiling.2026-01-01T00:00:00Z2026-01-01T02:02:03.17Z2025-01-27T20:39:24.852ZE-MTAB-14782ERP168680EFO_0002944EFO_0004170EFO_0005684EFO_0005518EFO_0003816EFO_0004184