biostudies-arrayexpressHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14791A549 OAS2 KO cells reconstituted with doxycycline-inducible OAS2 WT or OAS2 F524L were infected with EMCV and subjected to RNA-seq analysis to compare expression changes that are induced by OAS2 WT vs OAS2 F524L mutant.biostudies-arrayexpressSample Collection - Cells were seeded at a density 6250 cells/well in 1ml DMEM, FBS in 12 well plate. The next day cells were stimulated with 1ug/ml doxycycline for 3days. Afterwards cells were infected with EMCV MOI 0.3 or treated with media alone for 24h until cell harvest.Nucleic Acid Extraction - Cells were washed twice with PBS and lysed in the well with LBP buffer and RNA isolated following manufacturers instructions from Macherey Nagel NucleoSpin RNA Plus RNA isolation kit with gDNA removal.Sequencing - 150 base pairs paired-end sequencing on Illumina platformLibrary Construction - Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries were pooled and sequenced on Illumina platforms, according to effective library concentration and data amount.MINSEQE ScoreAssays and DataorganisationMAGE-TAB FilesMetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq XIlluminaRNA-seq of coding RNAHomo sapiensERP168736Indra BekereCarina Baer de Oliveira MannfalseRNA-seq of A549 OAS2 KO and KI cells infected with EMCVA549 OAS2 KO cells reconstituted with doxycycline-inducible OAS2 WT or OAS2 F524L were infected with EMCV and subjected to RNA-seq analysis to compare expression changes that are induced by OAS2 WT vs OAS2 F524L mutant.2025-05-22T00:00:00Z2026-06-05T19:55:29.735Z2025-01-29T13:26:11.687ZE-MTAB-14791ERP168736EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184