{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Shinkichi Ishikawa"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14793"],"description":["Human Pulmonary Alveolar Epithelial Cells (HPAEpiCs) purchased from ScienCell Research Laboratories were cultured at the air-liquid interface (ALI) for 28 days with medium containing Y-27632, A-83-01, and CHIR99021. The gene expression profile of HPAEpiCs was investigated on ALI day 0, 7, 14, 21, and 28 by RNA-seq."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit (Qiagen).","Sequencing - The library was sequenced on a NovaSeq 6000 (Illumina Inc.).","Library Construction - A DNA library was prepared using a TruSeq Stranded mRNA LT Sample Prep Kit (Illumina Inc.).","Sample Collection - HPAEpiCs were cultured at the ALI for 28 days with medium containing Y-27632, A-83-01, and CHIR99021. HPAEpiCs on ALI day 0, 7, 14, 21, and 28 were washed with PBS and lysed with Lysis buffer in Qiagen RNeasy Mini Kit (Qiagen)."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Shinkichi Ishikawa"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq analysis of HPAEpiCs Cultured at the ALI","description":"Human Pulmonary Alveolar Epithelial Cells (HPAEpiCs) purchased from ScienCell Research Laboratories were cultured at the air-liquid interface (ALI) for 28 days with medium containing Y-27632, A-83-01, and CHIR99021. The gene expression profile of HPAEpiCs was investigated on ALI day 0, 7, 14, 21, and 28 by RNA-seq.","dates":{"release":"2026-01-01T00:00:00Z","modification":"2026-01-01T02:02:03.165Z","creation":"2025-01-28T22:39:32.767Z"},"accession":"E-MTAB-14793","cross_references":{"ENA":["ERP168713"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}