biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsShinkichi IshikawaIllumina NovaSeq 6000RNA-seq of coding RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14793Human Pulmonary Alveolar Epithelial Cells (HPAEpiCs) purchased from ScienCell Research Laboratories were cultured at the air-liquid interface (ALI) for 28 days with medium containing Y-27632, A-83-01, and CHIR99021. The gene expression profile of HPAEpiCs was investigated on ALI day 0, 7, 14, 21, and 28 by RNA-seq.biostudies-arrayexpressNucleic Acid Extraction - Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit (Qiagen).Sequencing - The library was sequenced on a NovaSeq 6000 (Illumina Inc.).Library Construction - A DNA library was prepared using a TruSeq Stranded mRNA LT Sample Prep Kit (Illumina Inc.).Sample Collection - HPAEpiCs were cultured at the ALI for 28 days with medium containing Y-27632, A-83-01, and CHIR99021. HPAEpiCs on ALI day 0, 7, 14, 21, and 28 were washed with PBS and lysed with Lysis buffer in Qiagen RNeasy Mini Kit (Qiagen).OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesShinkichi IshikawafalseRNA-seq analysis of HPAEpiCs Cultured at the ALIHuman Pulmonary Alveolar Epithelial Cells (HPAEpiCs) purchased from ScienCell Research Laboratories were cultured at the air-liquid interface (ALI) for 28 days with medium containing Y-27632, A-83-01, and CHIR99021. The gene expression profile of HPAEpiCs was investigated on ALI day 0, 7, 14, 21, and 28 by RNA-seq.2026-01-01T00:00:00Z2026-01-01T02:02:03.165Z2025-01-28T22:39:32.767ZE-MTAB-14793ERP168713EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184