{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Dr Rupesh Dash"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14795"],"description":["In this study, we performed a CRISPR-based kinome screening in multiple cancer cell lines and identified Never In Mitosis Gene-A Related Kinase-9 (NEK9) as a major player of Docetaxel resistance in OSCC, prostate, and pancreatic cancer lines. We wanted to perform RNA sequencing to understand the possible mechanistic reason for drug sensitization."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - Total RNA was isolated using Direct-Zol RNA miniprep (R2052) RNA isolation kit.","Sequencing - Library cDNAs were sequenced in NovaSeq 6000 Sequencing System","Library Construction - To prepare an RNA-Sequencing library, 500ng of total RNA was used to isolate mRNA using magnetic beads (PolyA mRNA Isolation Module, NEB Cat No# E7490L). The RNA-Seq library was then prepared using an mRNA library preparation kit (NEB Cat No# E7770L) strictly following the vendor’s recommended protocol. Following library preparation, the library concentration was measured using Qubit 2.0 (Invitrogen), and the appropriate fragmentation sizes were confirmed using Bio-analyzer (Agilent Cat No# 5067-5582).","Sample Collection - DU145 NEK9 WT and KO cells were cultured in cell culture conditions in DMEM (10%FBS) . Cells were trypsinized and subjected to RNA isolation."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Dr Rupesh Dash"],"additional_accession":[]},"is_claimable":false,"name":"RNA sequencing of DU145 (a human prostate cancer cell line) NEK9 genetically Knocked out cell line compared to its wildtype parental counterpart","description":"In this study, we performed a CRISPR-based kinome screening in multiple cancer cell lines and identified Never In Mitosis Gene-A Related Kinase-9 (NEK9) as a major player of Docetaxel resistance in OSCC, prostate, and pancreatic cancer lines. We wanted to perform RNA sequencing to understand the possible mechanistic reason for drug sensitization.","dates":{"release":"2025-10-01T00:00:00Z","modification":"2025-10-01T01:06:00.384Z","creation":"2025-01-29T17:29:45.655Z"},"accession":"E-MTAB-14795","cross_references":{"ENA":["ERP168783"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}