biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsDr Rupesh DashIllumina NovaSeq 6000RNA-seq of coding RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14795In this study, we performed a CRISPR-based kinome screening in multiple cancer cell lines and identified Never In Mitosis Gene-A Related Kinase-9 (NEK9) as a major player of Docetaxel resistance in OSCC, prostate, and pancreatic cancer lines. We wanted to perform RNA sequencing to understand the possible mechanistic reason for drug sensitization.biostudies-arrayexpressNucleic Acid Extraction - Total RNA was isolated using Direct-Zol RNA miniprep (R2052) RNA isolation kit.Sequencing - Library cDNAs were sequenced in NovaSeq 6000 Sequencing SystemLibrary Construction - To prepare an RNA-Sequencing library, 500ng of total RNA was used to isolate mRNA using magnetic beads (PolyA mRNA Isolation Module, NEB Cat No# E7490L). The RNA-Seq library was then prepared using an mRNA library preparation kit (NEB Cat No# E7770L) strictly following the vendor’s recommended protocol. Following library preparation, the library concentration was measured using Qubit 2.0 (Invitrogen), and the appropriate fragmentation sizes were confirmed using Bio-analyzer (Agilent Cat No# 5067-5582).Sample Collection - DU145 NEK9 WT and KO cells were cultured in cell culture conditions in DMEM (10%FBS) . Cells were trypsinized and subjected to RNA isolation.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesDr Rupesh DashfalseRNA sequencing of DU145 (a human prostate cancer cell line) NEK9 genetically Knocked out cell line compared to its wildtype parental counterpartIn this study, we performed a CRISPR-based kinome screening in multiple cancer cell lines and identified Never In Mitosis Gene-A Related Kinase-9 (NEK9) as a major player of Docetaxel resistance in OSCC, prostate, and pancreatic cancer lines. We wanted to perform RNA sequencing to understand the possible mechanistic reason for drug sensitization.2025-10-01T00:00:00Z2025-10-01T01:06:00.384Z2025-01-29T17:29:45.655ZE-MTAB-14795ERP168783EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184