biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsMaxim MessererNextSeq 500RNA-seq of coding RNAChenopodium quinoaChenopodium quinoahttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14806Guard cells of quinoa were treated with salt and ABA to analyze expression changes.biostudies-arrayexpressNucleic Acid Extraction - Guard cells were enriched using the blender method (Bauer et al., 2013) and DNase I treated.Sequencing - Equimolar amounts of each library were sequenced on a NextSeq 500 instrument controlled by the NextSeq Control Software (NCS) v2.2.0, using one 150 Cycles High Output and one 150 Cycles Mid Output Kit with the single index, paired-end (PE) run parameters.Growth Protocol - Chenopodium quinoa (cv.5020) plants were cultivated in a greenhouse (at 22°C / 20°C day / night under a 16h light regime at 300 µmol m-2 s-1 white light) using common potting soil. NaCl- treatment was started after 2 weeks of growth. The NaCl concentration in the irrigation water started at 25 mM NaCl and was increased stepwise by doubling the concentration every second day up to 200 mM or 500 mM. After 5 weeks of salt treatment plants were used for experiments. For ABA stimulation of guard cells, ABA (50 µM ± ABA, Sigma) was fed to the stomata via the petiole of detached leaves, as the dense epidermal bladder growth prevented the usual spray treatment.Sample Collection - Guard cells were enriched using the blender method (Bauer et al., 2013).Library Construction - Library preparation and RNAseq were carried out as described in the Illumina TruSeq Stranded mRNA Sample Preparation Guide, the Illumina NextSeq 500 System Guide (Illumina, Inc., San Diego, CA, USA), and the KAPA Library Quantification Kit - Illumina/ABI Prism User Guide (Kapa Biosystems, Inc., Woburn, MA, USA).OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesMaxim MessererfalseGuard cells of Chenopodium quinoa treated with salt and ABAGuard cells of quinoa were treated with salt and ABA to analyze expression changes.2025-11-21T00:00:00Z2025-11-21T02:02:42.656Z2025-02-03T21:12:15.116ZE-MTAB-14806ERP168914EFO_0002944EFO_0004170EFO_0003789EFO_0005518EFO_0003738EFO_0004184