{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Sandrine Hughes"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14810"],"description":["The study compared two groups of mice first treated to become hypothyroid. The hypothalamus samples collected from each group of mice were pooled (n=4) and nuclei were sorted to prepare two libraries: one group was treated by thyroid hormones to restore normal TH levels, and the other group was left untreated. The snRNAseq experiment was performed on sorted single nuclei extracted from the dissected mediobasal hypothalamus to identify TH-responsive genes in the mouse hypothalamus."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - None - Included in nucleic acid library construction protocol","Sequencing - NextSeq500 Illumina sequencer - Paired-end High sequencing run","Library Construction - Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1; Dual Index","Sample Collection - The filtrated nuclei suspension was run through a cell sorter (Aria IIµ cytometer; BD Biosciences) to isolate 200 000 nuclei from each sample"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - Data were analysed with Seurat toolkit v5, using SCT normalization.","Sequence Alignment - Reads were analysed with the Cell Ranger pipeline (v7.1.0, 10X Genomics)  using the mouse genome (GRCm38 release 102)"],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["NextSeq 500"],"study_type":["single nucleus RNA sequencing"],"species":["Mus musculus"],"pubmed_title":["An atlas of thyroid hormone responsive genes in adult mouse hypothalamus"],"pubmed_authors":["Sandrine Hughes","Shijia Wu, Julien Dellinger, Suzy Markossian, Romain Guyot, Sandrine Hughes, Denise Aubert, Karine Gauthier, Benjamin Gillet, Wenzheng Jiang, Frédéric Flamant"],"additional_accession":[]},"is_claimable":false,"name":"snRNA-seq in adult mouse hypothalamus to identify genes responding to thyroid hormone","description":"The study compared two groups of mice first treated to become hypothyroid. The hypothalamus samples collected from each group of mice were pooled (n=4) and nuclei were sorted to prepare two libraries: one group was treated by thyroid hormones to restore normal TH levels, and the other group was left untreated. The snRNAseq experiment was performed on sorted single nuclei extracted from the dissected mediobasal hypothalamus to identify TH-responsive genes in the mouse hypothalamus.","dates":{"release":"2025-10-28T00:00:00Z","modification":"2025-10-28T10:41:42.31Z","creation":"2025-02-03T23:09:54.311Z"},"accession":"E-MTAB-14810","cross_references":{"ENA":["ERP168925"],"EFO":["EFO_0002944","EFO_0004170","EFO_0004917","EFO_0009809","EFO_0005518","EFO_0003816","EFO_0004184"]}}