{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Marek Marzec"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"organism":["Hordeum vulgare"],"species":["Hordeum vulgare"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14857"],"description":["A drought experiment was carried out on three barley genotypes: Sebastian (WT), hvd53a.f (strigolactone repressor) and hvd14.d (strigolactone receptor)."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - Samples were frozen immediately in liquid nitrogen; RNA was isolated using the mirVana miRNA Isolation Kit (Sigma, catalogue number: AM1560).","Sequencing - Transcriptome sequencing was conducted by Novogene Co., LTD (Beijing, China).","Growth Protocol - Plants were grown in a greenhouse for 10 days after sowing (DAS) under optimal water conditions (14% vwc), 20/18°C day/night, 16/8 photoperiod and 420 μE m−2 s−1 light intensity, in boxes (400 × 140 × 175 mm) filled with soil containing a mixture of sandy loam and sand (7:2) supplied with a nutrient medium. On 15 DAS, plants were moved into a growth chamber, where the temperature regime was set to 25°C/20°C day/night, with a 16/8 hr photoperiod and 420 μEm−2 s−1 light intensity","Sample Collection - For RNA-isolation analyses, plant tissue (leaf) was collected in four biological replicates, each containing tissue from 3 seedlings.","Library Construction - Preparation of RNA library was conducted by Novogene Co., LTD (Beijing, China)."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Marek Marzec"],"additional_accession":[]},"is_claimable":false,"name":"Response to drought of three barley genotypes: Sebastian (WT), hvd53a.f (strigolactone repressor) and hvd14.d (strigolactone receptor) compared to the corresponding plants growing in control conditions","description":"A drought experiment was carried out on three barley genotypes: Sebastian (WT), hvd53a.f (strigolactone repressor) and hvd14.d (strigolactone receptor).","dates":{"release":"2026-09-30T00:00:00Z","modification":"2026-09-30T01:00:35.524Z","creation":"2025-02-18T10:40:23.005Z"},"accession":"E-MTAB-14857","cross_references":{"ENA":["ERP169310"],"EFO":["EFO_0002944","EFO_0004170","EFO_0003789","EFO_0005518","EFO_0003738","EFO_0004184"]}}