biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsMarek MarzecIllumina NovaSeq 6000RNA-seq of coding RNAHordeum vulgareHordeum vulgarehttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14857A drought experiment was carried out on three barley genotypes: Sebastian (WT), hvd53a.f (strigolactone repressor) and hvd14.d (strigolactone receptor).biostudies-arrayexpressNucleic Acid Extraction - Samples were frozen immediately in liquid nitrogen; RNA was isolated using the mirVana miRNA Isolation Kit (Sigma, catalogue number: AM1560).Sequencing - Transcriptome sequencing was conducted by Novogene Co., LTD (Beijing, China).Growth Protocol - Plants were grown in a greenhouse for 10 days after sowing (DAS) under optimal water conditions (14% vwc), 20/18°C day/night, 16/8 photoperiod and 420 μE m−2 s−1 light intensity, in boxes (400 × 140 × 175 mm) filled with soil containing a mixture of sandy loam and sand (7:2) supplied with a nutrient medium. On 15 DAS, plants were moved into a growth chamber, where the temperature regime was set to 25°C/20°C day/night, with a 16/8 hr photoperiod and 420 μEm−2 s−1 light intensitySample Collection - For RNA-isolation analyses, plant tissue (leaf) was collected in four biological replicates, each containing tissue from 3 seedlings.Library Construction - Preparation of RNA library was conducted by Novogene Co., LTD (Beijing, China).OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesMarek MarzecfalseResponse to drought of three barley genotypes: Sebastian (WT), hvd53a.f (strigolactone repressor) and hvd14.d (strigolactone receptor) compared to the corresponding plants growing in control conditionsA drought experiment was carried out on three barley genotypes: Sebastian (WT), hvd53a.f (strigolactone repressor) and hvd14.d (strigolactone receptor).2026-09-30T00:00:00Z2026-09-30T01:00:35.524Z2025-02-18T10:40:23.005ZE-MTAB-14857ERP169310EFO_0002944EFO_0004170EFO_0003789EFO_0005518EFO_0003738EFO_0004184