{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":[null],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14860"],"description":["Analysis of 40S ribosome profiling of Eif6 WT and Eif6 S235A MEFs"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - Immortalized MEF cells were washed once with ice-cold PBS 10ug/ml cycloheximide.","Library Construction - Immagina Biotechnology LACEseq #LS-001_12","Nucleic Acid Extraction - Cells were lysed in 20mM Tris-HCl, 50mM NaCl, 10mM MgCl2, 1mM DTT, 100ug/ml cyclohexamide, 1% NP-40, 0.2mg/ml heparin. Ribosome Protected Fragments (RPF) were obtained following Ingolia 2017 (10.1016/j.ymeth.2017.05.028).  Following RNase digestion 40S were purified on 10-50% sucrose gradients.","Sequencing - 500 pM library, Phix 10%, NextSeq 1000/2000 P2 100 cycles Illumina Kit, sequenced with NextSeq 2000"],"figure_sub":["MINSEQE Score","Assays and Data","organisation","MAGE-TAB Files"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["NextSeq 2000"],"study_type":["Ribo-seq"],"species":["Mus musculus"],"additional_accession":["ERP169380"],"pubmed_authors":["Ivan Ferrari"]},"is_claimable":false,"name":"40S footprints of Eif6 WT and Eif6 S235A MEFs","description":"Analysis of 40S ribosome profiling of Eif6 WT and Eif6 S235A MEFs","dates":{"release":"2026-02-10T00:00:00Z","modification":"2026-02-10T09:34:07.712Z","creation":"2025-02-21T10:07:00.547Z"},"accession":"E-MTAB-14860","cross_references":{"ENA":["ERP169380"],"EFO":["EFO_0002944","EFO_0004170","EFO_0008891","EFO_0005518","EFO_0004184"]}}