biostudies-arrayexpressMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14860Analysis of 40S ribosome profiling of Eif6 WT and Eif6 S235A MEFsbiostudies-arrayexpressSample Collection - Immortalized MEF cells were washed once with ice-cold PBS 10ug/ml cycloheximide.Library Construction - Immagina Biotechnology LACEseq #LS-001_12Nucleic Acid Extraction - Cells were lysed in 20mM Tris-HCl, 50mM NaCl, 10mM MgCl2, 1mM DTT, 100ug/ml cyclohexamide, 1% NP-40, 0.2mg/ml heparin. Ribosome Protected Fragments (RPF) were obtained following Ingolia 2017 (10.1016/j.ymeth.2017.05.028). Following RNase digestion 40S were purified on 10-50% sucrose gradients.Sequencing - 500 pM library, Phix 10%, NextSeq 1000/2000 P2 100 cycles Illumina Kit, sequenced with NextSeq 2000MINSEQE ScoreAssays and DataorganisationMAGE-TAB FilesMetabolomicsUnknownTranscriptomicsGenomicsProteomicsNextSeq 2000Ribo-seqMus musculusERP169380Ivan Ferrarifalse40S footprints of Eif6 WT and Eif6 S235A MEFsAnalysis of 40S ribosome profiling of Eif6 WT and Eif6 S235A MEFs2026-02-10T00:00:00Z2026-02-10T09:34:07.712Z2025-02-21T10:07:00.547ZE-MTAB-14860ERP169380EFO_0002944EFO_0004170EFO_0008891EFO_0005518EFO_0004184