{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":[null],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14864"],"description":["Analysis of global variability of translation in all conditions. Ribosome Protected Fragments (RPFs) were obtained following the procedure reported in McGlincy NJ & Ingolia NT. Methods. 2017 (doi: 10.1016/j.ymeth.2017.05.028). Samples labelled with \\\"HS\\\" were treated with 0.5M of KCl before digestion."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - Immortalized MEF cells were washed once with ice-cold PBS 10ug/ml cycloheximide.","Library Construction - Immagina Biotechnology LACEseq #LS-001_12","Sample Treatment - treatment with 0.5M of KCl before digestion","Nucleic Acid Extraction - Cells were lysed in 20mM Tris-HCl, 50mM NaCl, 10mM MgCl2, 1mM DTT, 100ug/ml cycloheximide, 1% NP-40, 0.2mg/ml heparin. Ribosome Protected Fragments (RPF) were obtained following Ingolia 2017 (10.1016/j.ymeth.2017.05.028).","Sequencing - 500 pM library, Phix 10%, NextSeq 1000/2000 P2 100 cycles Illumina Kit, sequenced with NextSeq 2000","Sample Treatment - digestion without KCl treatment"],"figure_sub":["MINSEQE Score","Assays and Data","organisation","MAGE-TAB Files"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["NextSeq 2000"],"study_type":["Ribo-seq"],"species":["Mus musculus"],"additional_accession":["ERP169393"],"pubmed_authors":["Ivan Ferrari"]},"is_claimable":false,"name":"Ribo-seq of Eif6 WT, Eif6 S235A, Eif6 S235A rescued  with eIF6WT and high salt treated MEFs","description":"Analysis of global variability of translation in all conditions. Ribosome Protected Fragments (RPFs) were obtained following the procedure reported in McGlincy NJ & Ingolia NT. Methods. 2017 (doi: 10.1016/j.ymeth.2017.05.028). Samples labelled with \\\"HS\\\" were treated with 0.5M of KCl before digestion.","dates":{"release":"2026-02-10T00:00:00Z","modification":"2026-02-10T09:33:12.418Z","creation":"2025-02-21T15:35:49.506Z"},"accession":"E-MTAB-14864","cross_references":{"ENA":["ERP169393"],"EFO":["EFO_0002944","EFO_0004170","EFO_0008891","EFO_0005518","EFO_0004184","EFO_0003969"]}}