{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Kassandra Carpio"],"organism":["West Nile virus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-14867"],"description":["West Nile infectious clones were transfected with either SLA or tRNA. Viral RNA was extracted on days 3 and 4 post-transfection for sequencing. Shannon entropy and single nucleotide variants were analyzed to compare the samples."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - cell culture supernatant","Nucleic Acid Extraction - QIAamp Viral RNA Mini Kit","Sequencing - samples sequenced on the Illumina NextSeq 550 instrument","Library Construction - cDNA libraries were prepared using random hexamer primers","Sample Treatment - viral RNA was transfected with SLA or tRNA"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"data_protocol":["Sequence Alignment - reads were aligned using Bowtie2 to a reference genome"],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["NextSeq 550"],"study_type":["RNA-seq of coding RNA"],"species":["West Nile virus"],"pubmed_title":["Interaction of West Nile virus NS5 with orthoflavivirus SLA RNAs and their effects on viral replication and inhibition"],"pubmed_authors":["Mandi A. Feinberg, My T. Le, Kassandra L. Carpio, Ekaterina Knyazhanskaya, Alan D. Barrett, Kyung (Kay) H. Choi","Kassandra Carpio"],"additional_accession":[]},"is_claimable":false,"name":"NGS of West Nile viruses replicating in the presence of SLA RNAs or tRNA controls","description":"West Nile infectious clones were transfected with either SLA or tRNA. Viral RNA was extracted on days 3 and 4 post-transfection for sequencing. Shannon entropy and single nucleotide variants were analyzed to compare the samples.","dates":{"release":"2025-07-15T00:00:00Z","modification":"2025-07-15T09:31:27.999Z","creation":"2025-02-21T15:39:29.585Z"},"accession":"E-MTAB-14867","cross_references":{"ENA":["ERP169395"],"EFO":["EFO_0002944","EFO_0004170","EFO_0004917","EFO_0005518","EFO_0003738","EFO_0004184","EFO_0003969"]}}