biostudies-arrayexpressKassandra CarpioWest Nile virushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-14867West Nile infectious clones were transfected with either SLA or tRNA. Viral RNA was extracted on days 3 and 4 post-transfection for sequencing. Shannon entropy and single nucleotide variants were analyzed to compare the samples.biostudies-arrayexpressSample Collection - cell culture supernatantNucleic Acid Extraction - QIAamp Viral RNA Mini KitSequencing - samples sequenced on the Illumina NextSeq 550 instrumentLibrary Construction - cDNA libraries were prepared using random hexamer primersSample Treatment - viral RNA was transfected with SLA or tRNAOrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesSequence Alignment - reads were aligned using Bowtie2 to a reference genomeUnknownTranscriptomicsGenomicsProteomicsNextSeq 550RNA-seq of coding RNAWest Nile virusInteraction of West Nile virus NS5 with orthoflavivirus SLA RNAs and their effects on viral replication and inhibitionMandi A. Feinberg, My T. Le, Kassandra L. Carpio, Ekaterina Knyazhanskaya, Alan D. Barrett, Kyung (Kay) H. ChoiKassandra CarpiofalseNGS of West Nile viruses replicating in the presence of SLA RNAs or tRNA controlsWest Nile infectious clones were transfected with either SLA or tRNA. Viral RNA was extracted on days 3 and 4 post-transfection for sequencing. Shannon entropy and single nucleotide variants were analyzed to compare the samples.2025-07-15T00:00:00Z2025-07-15T09:31:27.999Z2025-02-21T15:39:29.585ZE-MTAB-14867ERP169395EFO_0002944EFO_0004170EFO_0004917EFO_0005518EFO_0003738EFO_0004184EFO_0003969